A1 GLP

Question 1

A facility of science that is equipped for different experiments, testing, and analysis.

Answer 1

laboratory

Question 2

General Laboratory Safety Precautions:

Answer 2

1. Wearing appropriate PPE is important.
2. Keep working areas clean.
3. Understand the properties of the reagents and chemicals to be
   used.
4. Understand the biosafety procedures when asked to handle live biological specimens.
5. If you have no experience handling any equipment, make sure to have someone supervise you during their operations.
6. Inspect the electrical outlets, cords, and wiring before using.
7. wash your hands before and after laboratory work.
8. No use of smartphones during laboratory work to prevent distractions.

Question 3

Sample Handling and Contamination Prevention:

Answer 3

1. Reloading of tip boxes with tips or glass containers with microfuge tubes to be used in any nucleic acid workflow should be done in clean, disinfected tables.
2. When doing DNA, RNA, or plasmid workflows, make sure to use the designated pipets, pipet tips, microfuge tubes, etc.
3. Labels are important in prepared media or reagents!
4. Labels are more important in samples!
5. Handling of samples or reagents that are heat sensitive should always be kept on ice or in proper temperature-controlled conditions.
6. Know when to reduce and reuse tips during a MolBio lab experiment.
7. Making aliquots of stored sensitive reagents or samples is essential in preserving their integrity in the long run.

Question 4

Good Laboratory Records Keeping:

Answer 4

1. Label your laboratory notebooks.
2. Recording the date is essential.
3. Following the date should be the title of the activity to be implemented on that day.
4. Rewrite the protocol of the laboratory activity into a flowchart reflecting only the important steps to be conducted.
5. Any changes made during the experiment should also be recorded.
6. Document the results of the experiment.
7. Always keep your notebook up-to-date.
8. Strikethrough the mistaken entry or content with one line so that they may still be readable.
9. Whenever results are available and incorporated in the lab notebook, take time to digest their meaning.
10. At the end of every activity, write a reflection on the activity done.

Question 5

Proper Waste Management:

Answer 5

1. Observing proper segregation.
2. Waste containers should be chemically compatible with the waste.
3. Most of the chemicals used in the lab can undergo drain disposal.
4. Broken glassware or sharps should be appropriately discarded using puncture-proof containers.
5. Since some of the lab work would entail the use of live microorganisms either as samples or model organisms, disposing of them properly is also important.

Question 6

Compliance with Institutional Regulations:

Answer 6

1. Keep in mind the dos and don’ts.
2. Be familiar with the location and use of emergency equipment.
3. Ensure the availability of first aid kits and their use and location. Any accidents should be reported immediately to the lab instructor.
4. Know the location of the fire exits and routes in case of emergency.