A2 Basic Calculations Flashcards
buffer preparations are usually more tedious since the pH of the final solution is dependent on the ratio of the acid to its conjugate base.
Antibiotics, Vitamins, and Amino acids are thermolabile (heat-sensitive) = non-autoclavable
only up to 1% of concentrated stock can be considered negligible to the overall computations of media preparations. Anything more than this may affect the final concentration of the components in the media.
is a measure of the concentration of a solute in a solution and is defined as the number of moles of solute per liter of solution. It is expressed in moles per liter (mol/L) or M.
Molarity
The formula for molarity is:
M (š¦šØš„/š) = š¦šØš„šš¬ šØš š¬šØš„š®šš (š¦šØš„)/ššØš„š®š¦š šØš ššØš„š®šš¢šØš§ (š)
mass of the solute to be added when M is given
Mass of Solute = (Molarity x Volume of Solution) x Molar Mass
unlike molarity, ___________considers the number of equivalents (or eq, a measure of the reactive capacity of a substance) of solute per liter of solution
Normality
Formula for Normality:
N (ššŖ/š) = šš®š¦ššš« šØš ššŖš®š¢šÆšš„šš§šš¬ (šš)/ššØš„š®š¦š šØš ššØš„š®šš¢šØš§ (š)
a type of acids that are able to donate only one proton
monoprotic substances
produce more than 1 ion
per mole of solution
polyprotic substances
are solutions that help stabilize pH levels by resisting the effects of acids or bases.
Buffers
Henderson-Hasselbalch equation
pš = š©šš + š„šØš ([šā]/[šš])
