9/22- Cases Membranous, Crescentic, Post-infectious and Membranoproliferative GN Flashcards
Into what 3 categories can renal diseases be divided?
Diseases affecting:
- Glomeruli
- Tubulo-interstitium
- Vessels
Clinical presentations of:
- Nephrotic syndrome
- Nephritic syndrome
- Acute renal failure
- Chronic renal failure
Nephrotic syndrome
- Proteinuria, edema, hypoalbuminemia, hypercholesterolemia, lipiduria
Nephritic syndrome
- Hematuria, mild proteinuria, hypertension
Acute renal failure
- Oliguria or anuria, elevated BUN and creatinine
Chronic renal failure
- Chronic elevation of BUN and Creatinine
Case)
- A 56 year-old woman without any significant past medical history presented with progressive edema for the past two months
- An annual check-up 10 months ago showed no protein excretion and normal serum creatinine (0.7mg/dl)
- Currently, the serum creatinine was 1.1 mg/dl.
- There was 4+ proteinuria by dip stick and the 24-hour urine collection revealed 5 grams of protein.
- Urine analysis showed 5 RBCs and no WBCs/HPF.
- All serologic studies including ANA, anti-DNA, and viral hepatitis panel were negative.
- A renal biopsy was performed.
What renal syndrome does this pt have?
Why is a renal biopsy indicated?
NEPHROTIC syndrome.
The two most important elements of this syndrome:
- Proteinuria > 3 g/day
- Peripheral edema
(Other elements include: hypeprlipidemia, lipiduria, hypoalbuminemia; plasma albumin under 3 g/dL)
There are a variety of renal lesions that can cause nephrotic syndrome and a specific diagnosis is needed for proper management of this patient.
Protein loss implies disease at what level?
Glomerulus
- Abnormal filtration and excretion of proteins
What provides the negative charge on the glomerular filtration membrane?
- Heparan sulfate -> GBM (-)
- Sialoproteins -> podocyte/endothelium (-)
What are possible causes of nephrotic syndrome?
Many; limited number account for a majority
Pediatrics:
- Minimal change disease (#1 in kids)
- Focal segmental glomerulosclerosis (idiopathic)
Adults:
- Membranous glomerulonephropathy
- Focal segmental glomerulosclerosis (idiopathic)
- Diabetic nephropathy
- Amyloidosis
What is seen here?
Membranous glomerulopathy (inset = normal)
- Diffuse thickening of the glomerular capillaries
- Glomerular capillary loops appear thickened, eosinophilic and “stiff”
- Some capillary loops may actually appear “fuzzy” and special stains may shows a “spiked” GBM or “hair on end” appearance
- Epi- or intramembranous deposits (red dots) may be seen on Trichrome staining
- It is not uncommon to have increased mesangial matrix and even segmental lesions
What does the IF staining for IgG show in Membranous GN?
Global, diffuse, and granular staining for IgG along the glomerular capillaries
What do you see in the EM of membranous GN?
- Multiple subepithelial or intramembranous immune deposits
- Thickened basement membrane
- Effacement of foot processes
(Don’t confuse with “humps”; latter are more like haystacks)
What will show up on silver stain in membranous GN?
EM with subepithelial electron dense deposits, separated by spikes of basement membrane material.
- Silver stains basement membrane but not deposits
- Results in “hair on end”, spiked look
What are the different types of Membranous GN?
Primary: idiopathic
Secondary:
- Solid tumors*
- Systemic lupus
- HepB
- Some drugs
*Up to about 5-10% of cases of membranous glomerulonephropathy (esp in elderly) are associated with neoplasms
Treatment: membranous GN?
- Investigate for 2ndary causes
- then start on steroid therapy?
Case 2)
- A 62 year-old woman without a significant past medical history presented with sore throat, malaise, arthralgia, and weight loss
- Physical examination was unremarkable.
Lab findings:
- Serum creatinine 3.6 mg/dl
- Urine analysis 3+ blood, 1+ protein + P-ANCA 1:600; + MPO-ANCA 20U/dl; negative C-ANCA, ANA, anti-DNA antibody, anti-GBM antibody, hepatitis serology, rheumatoid factor; normal serum C3 and C4.
- A renal biopsy was performed.
What renal syndrome does this patient have?
Why was a renal biopsy performed in this patient?
NEPHRITIC syndrome
- Decreased renal function
- Hematuria (RBCs in urine)
- Classically: grossly visible hematuria, mild-mod proteinuria and HTN
Why renal biopsy?
- Possibility of a glomerulonephritis
- The positive ANCA suggests that the glomerulonephritis may be related to ANCA.
- However, there is still reason to perform the kidney biopsy in this patient
- ANCA may be positive in many unrelated conditions including inflammatory bowel disease, some types of hepatitis, and other non-renal immune-mediated diseases
- In addition, there are well-illustrated cases in which there is a discrepancy between the clinical presentations and the renal lesions revealed by kidney biopsy
- Therefore, the kidney biopsy findings serve not only to confirm the diagnosis of crescentic glomerulonephritis but also to determine how severe and how active the renal lesions are.
- These features are important for treatment decisions.
What is the underlying pathophysiology of nephritic syndrome?
Disruption of capillary loops with spillage of protein and red cells
- Active urinary sediment Spilled proteins may incite crescent formation
What is the significance of the laboratory findings, especially the serologic tests?
- Serum creatinine 3.6 mg/dl
- Urine analysis 3+ blood, 1+ protein
+ P-ANCA 1:600; + MPO-ANCA 20U/dl; negative C-ANCA, ANA, anti-DNA antibody, anti-GBM antibody, hepatitis serology, rheumatoid factor; normal serum C3 and C4.
Help with DDx for crescentic glomerulonephritis
- Negative ANA, anti-DNA, and anti-GBM and other negative serological tests help to rule out lupus nephritis, anti-GBM antibody mediated disease and viral hepatitis-related glomerular lesions
- Positive ANCA supports possibility of ANCA-related glomerulonephritis
What is ANCA?
AntiNeutrophilic Cytoplasmic Antibodies
- Mainly in primary granules of neutrohpils, but also lysosymes of monocytes and endothelial cells