9 -10 RBC diameter measurement and autoanalyzer

Question 1

how to calibrate microscope to measure rbc diameter

Answer 1

focus the ocular micrometer on the burker lines. see how many lines in 50 um. e.g. 10 lines in 50 um means each line is 5 um. if RBC is 3 lines then its 15 um

Question 2

automated hematology analyzer is based on what property of RBC and plasma

Answer 2

RBC is a bad conductor while plasma is a good conductor

Question 3

what happens when a cell passes through the aperture

Answer 3

it acts as an insulator and causes resistance to current flow and we get voltage spike.

Question 4

what is the size of the voltage spike proportional to

Answer 4

to the size of the cell that flows through the aperture

Question 5

how do we use the voltage spikes to calculate the cell count

Answer 5

number of spikes in a period of time is proportional to the number of the cells in the sample

Question 6

how do we meaure RBC and platelets automatically

Answer 6

together. we know the difference between them due to the difference in voltage spike since platelets are much smaller they give smaller spike. in addition, we subtract the number of WBC from RBC

Question 7

WBC automated count?

Answer 7

we hemolyze the RBC and do the procedure

Question 8

hemoglobin automated count?

Answer 8

based on photometry