8.2 Recombinant DNA, PCR & Electrophoresis

Question 1

Restriction enzymes have enabled scientists to _____

Answer 1

cut DNA into smaller, more usable fragments and isolate single genes.

Question 2

What are the two types of restriction enzymes?

Answer 2

Sticky-end restriction enzymes

Blunt-end restriction enzymes

Question 3

Sticky-end restriction enzymes?

Answer 3

Cut DNA at different locations in the recogniton site, leaving DNA fragments with overhanging ends.

Question 4

Blunt-end restriction enzymes?

Answer 4

Leaves clean cuts by cutting through the sugar phosphate backbone on both strands of the DNA molecule at the same location with in the recognition site.

Question 5

What are Ligases?

Answer 5

A group of enzymes that joins fragments of DNA or RNA together through a process called ligation.