8. post transcriptional regulation of gene expression Flashcards
1
Q
What are the 3 important processing steps of the primary RNA transcript in eukaryotes?
A
5’ capping
RNA splicing
3’ polyadenylation
2
Q
Why are the processing steps important?
A
mRNA stability
translation
protein function
3
Q
What is crucial for mRNA processing proteins?
A
CTD phosphorylation of RNA pol II
4
Q
Which enzymes are involved in 5’ capping?
A
Phosphatase
Guanyl transferase
Methyl transferase
Methyl transferase
5
Q
what are the crucial proteins for polyadenylation?
A
CPSF
CstF
PAP
PABP
6
Q
What is AAUAAA bound by in polyadenylation?
A
CPSF
7
Q
What is GU bound by in polyadenylation?
A
CstF
8
Q
What is CA bound by in polyadenylation?
A
CFII
9
Q
What kind of reaction is splicing?
A
Two step transesterification
10
Q
What makes up the spliceosome?
A
5 snRNPs and more than 200 proteins
