7. eukaryotic transcription 2 Flashcards
1
Q
What can be used to analyse expression of a single gene and what do they tell us? 3 methods
A
RT-PCR - where and when
Live cell imaging - where and when
Promoter studies - where when why
2
Q
What can be used to analyse expression of all genes and what does it tell us? 2 methods
A
Microarrays - when where why
RNA sequencing - where when why
3
Q
What reporter genes can be added in next to the promotor sequence in live cell imaging and what colours are shown?
A
Lac Z - blue
GFP - green
4
Q
What does RNA sequencing allow the identification of?
A
gene splicing variants
