8 - DNA, Genes and Protein Synthesis Flashcards
gene
section of DNA located at a particular locus on a DNA molecule that contains the coded information for the amino acid sequences of polypeptides and functional RNA
degenerate code
most amino acids are coded for by more than one triplet
universal code
each triplet codes for the same amino acid in all organisms
exons
coding sequences in eukaryotic DNA
introns
non-coding sequences in eukaryotic DNA
eukaryotic DNA
has histone proteins associated with it
long and linear molecules
prokaryotic DNA
not associated with proteins
shorter and circular
homologous pair
two chromosomes that carry the same genes, but not necessarily the same alleles of the genes
allele
a form of a gene
mRNA
messenger RNA, used to transfer sections of DNA from the nucleus to the cytoplasm because it is small enough to fit through nuclear pores
long stranded polynucleotide arranged in a single helix
codon
sequence of 3 bases on mRNA that codes for a single amino acid
genome
complete set of genes in a cell
proteome
full range of proteins produced by the genome
tRNA
small, single-stranded polynucleotide consisting of about 80 nucleotides
folded into a clover leaf shape
carries a specific amino acid
transcription
- DNA helicase acts on a specific region of DNA, causing the DNA strands to separate and expose the nucleotide bases in that region
- one of the DNA strands acts as a template strand and pairs with free complementary RNA nucleotides
- RNA polymerase moves along the strand and joins the RNA nucleotides together to form a pre-mRNA molecule
- the DNA strand rejoins behind the RNA polymerase
- when a stop code is reached, RNA polymerase detaches and a section of pre-mRNA is produced
splicing
process of removing introns from pre-mRNA to leave only the coding exons
produces mRNA
occurs in eukaryotic cells
translation
- a ribosome becomes attached to the starting codon at one end of the mRNA molecule
- the ribosome works along the mRNA bringing together tRNA molecules with complementary anticodons to the mRNA codons
- the tRNA molecules each have a specific amino acid attached to them
- the amino acids attached to the tRNA molecules are joined by peptide bonds
- these form a polypeptide chain