6. Nutrition, cultivation, growth Flashcards

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1
Q

Describe macronutrients

A

Elements required in fairly large amounts

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2
Q

Describe micronutreinets

A

Metals needed in very small amounts

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3
Q

Describe growth factors

A

Organic compounds needed in very small amounts

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4
Q

What is a heterotroph?

A

Uses organic carbon sources - presynthesized by someone else

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5
Q

What is a autotroph?

A

Uses an inorganic carbon form, e.g. CO2

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6
Q

What is a photolito- and photoorganotroph.

A

Both use light as energy form.

Lito=inorganic (plants, algae)

Organo=organic (certain bacteria)

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7
Q

What is a chemolitotroph and a chemoorganotroph?

A

Uses chemicals as energy form

lito=inorganic (certain bacteria)

organo=organic (most bacteria and fungi)

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8
Q

Describe the way of using H, HS, S, NH4, NO2- and Fe2+ as electron donor.

A

H2 + 1/2 O2 -> H20

HS- + H+ 1/2 O2 -> S0 + H20

S0 + 3/2 O2 + H2O -> SO4 2- + 2H+

NH4+ + 3/2 O2 + H2O -> NO2- + 2H+

NO2- + 1/2 O2 -> NO3-

Fe2+ + H+ 1/4 O2 -> Fe3+ + 1/2 H2O

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9
Q

Describe catabolism and anabolism.

A

Catabolism is energy “yielding”

Anabolism is energy consuming.

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10
Q

What are the types of work (4) microorganism do?

A

1) flagellar movements.
2) Transport molecules against concentration gradients – uptake.
3) Maintenance – non-growth related reactions and processes such as: keep optimal concentration of metabolites and ions…, building and maintaining turgor pressure.
4) Drive otherwise energetically unfavourable reactions (via ATP)

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11
Q

What is ATP used for?

A

Carrier of available, useful energy. All energy requiring processes in a cell directly or indirectly coupled to ATP hydrolysis. Mobile: the cell’s energy currency. Central role between catavolism and anabolism. Substrate in signal transduction pathways by kinases that phosphorylate proteins and lipids. Phosphorylation agent, gives activated intermediary.

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12
Q

Why is iron important?

A

Iron – major role in cellular respiration (electron transport), key components of cytochromes and iron-sulphur proteins.
To obtain iron from various insoluble minerals, cells produce agents called siderophores – bind iron and transport it into the cell.

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13
Q

What is aquachelin?

A

Many marine siderophores are surface-active and tend to form molecular aggregates, for example aquachelins. The presence of the fatty acyl chain renders the molecules with a high surface activity and an ability to form micelles.[34] Thus, when secreted, these molecules bind to surfaces and to each other, thereby slowing the rate of diffusion away from the secreting organism and maintaining a relatively high local siderophore concentration

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14
Q

What is generation time?

A

The time it takes for a microorganism to increase one generation.

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15
Q

What is my?

A

My is the specific growth rate.

my=ln2/G

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16
Q

How can the amount of biomass be calculated?

A

dX/dt=my*X