5A & B: Energy transfers Flashcards
Photosynthesis, respiration, energy transfers & nutrient cycles
What do plants need energy for?
Photosynthesis, active transport (roots), DNA replication, cell division and protein synthesis
What do animals need energy for?
Muscle contraction, maintenance of body temp, active transport, DNA replication, cell division and protein synthesis
What is the overall equation for photosynthesis?
6CO2 + 6H2O + energy –> C6H12O6 + 6O2
What is the overall equation for aerobic respiration?
C6H12O6 + 6O2 –> 6CO2 + 6H2O + energy
In respiration the energy released from glucose is used to make what molecule?
ATP
What is a metabolic pathway?
A series of small reactions controlled by enzymes e.g respiration and photosynthesis
What is phosphorylation?
Adding a phosphate to a molecule e.g ADP is phosphorylation to ATP
What is photophosphorylation?
Adding a phosphate using light
What is photolysis?
The splitting of a molecule using light energy
What is photoionisation?
When light ‘excites’ the electrons in an atom/ molecule, giving them more energy and causing them to be released. The release of electrons causes the atom/molecule to become a positive ion
What is hydrolysis?
The splitting of a molecule using water
What is decarboxylation?
The removal of a carboxyl group from a molecule - Co₂ is released
What is dehydrogenation?
The removal of a hydrogen from a molecule
What are redox reactions?
Reactions that involve oxidation and reduction
What happens in a reduction reaction?
Reduction = it has gained electrons, and may have gained hydrogen or lost oxygen
What happens in an oxidation reaction?
Oxidation = it has lost electrons, and may have lost hydrogen or gained oxygen
Mnemonic to remember redox reactions?
OIL RIG
Oxidation Is Loss, Reduction Is Gain
What is a coenzyme?
A molecule that aids the function of an enzyme
What is the coenzyme used in photosynthesis?
NADP
What does NADP do?
NADP transfers hydrogen from one molecule to another - means it can reduce (give hydrogen) or oxidise (take hydrogen from) a molecule
Which coenzymes are used in respiration?
NAD, FAD and coenzyme A
What do NAD and FAD do?
NAD and FAD transfer hydrogen from one molecule to another - means they can reduce (give hydrogen) or oxidise (take hydrogen from) a molecule
What does Coenzyme A do?
Transfers acetate between molecules
Where does photosynthesis take place?
In the chloroplasts
What wavelength does PSI best absorb light at?
PSI = 700 nm
What wavelength does PSII best absorb light at?
PSII = 680 nm
Where does the light dependent reaction take place?
The thylakoid membrane
What is the 1st stage of the light dependent reaction called?
Stage 1: Photoionisation
Describe stage 1 of the light dependent reaction. (photoionisation)
- Light energy is absorbed by PSII
- Light excites the electrons in chlorophyll
- The electrons move to a higher energy level
- These high-energy electrons are released from the chlorophyll and move down the electron transport chain to PSI
Describe stage 2 of the light dependent reaction. (photolysis of water)
- As the excited electrons from chlorophyll leave PSII to move down the electron transport chain, they must be replaced
- Light energy splits water into protons (H+), electrons and oxygen (water is oxidised)
- The reaction is H20 –> 2H+ + 1/2 O2
What is the 2nd stage of the light dependent reaction called?
Photolysis
Describe stage 3 of the light dependent reaction.
- The excited electrons lose energy as they move down the electron transport chain
- This energy is used to transport H+ ions across the thylakoid membrane (into the thylakoid), so that the thylakoid has a higher conc. of H+ than the stroma - forms a proton gradient across the thylakoid membrane
- H+ move down their gradient, into the stroma via ATP synthase
- The energy from this movement allows ATP synthase to combine ADP + Pi to form ATP
What is chemiosmotic theory?
The process of electrons flowing down the electron transport chain and creating a proton gradient across the membrane to drive ATP synthase to form ATP
What is the light independent reaction also known as?
The Calvin cycle
Where does the light independent reaction take place?
In the stroma of the chloroplasts
What does the light independent reaction produce?
It produces triose phosphate (TP)
What can triose phosphate also be used for?
Can be used to make carbohydrates e.g glucose
Hexose sugars are made by joining two TP molecules together
What does the calvin cycle need to keep it going?
ATP and H+ ions
Describe the 1st stage of the Calvin cycle
Ribulose bisphosphate is combined with CO2 (enters leaf via stomata and diffuses into the stomata) by enzyme Rubisco, which produces an unstable 6C molecule which quickly breaks down into 2 glycerate 3-phosphate molecules (3C)
How many carbons does ribulose bisphosphate have?
5 carbons
What enzyme catalyses the combination of CO2 and ribulose bisphosphate?
Rubisco
Describe the 2nd stage of the Calvin cycle
- The hydrolysis of ATP (from LDR) provides energy to turn GP into triose phosphate (TP) which has 3 carbons.
- This reaction also requires H+ ions (from LDR), which come from NADPH. NADPH is converted into NADP
- Some (1/6) TP is converted into useful organic compounds e.g glucose
How many molecules of glycerate 3-phosphate are produced from ribulose bisphosphate?
2 GP molecules are produced from 1 RuBP
NADPH ______ GP to TP, as a result NADPH is _____
NADPH reduces GP to TP, as a result NADPH is oxidised
Describe the 4th stage of the Calvin cycle
- 5/6 TP molecules are used to regenerate RuBP
- Regenerating RuBP uses the rest of the ATP produced in the LDR
How many carbons does TP have?
TP has 3 carbons
What does the Light dependent reaction produce?
ATP and reduced NADP
Name 3 photosynthetic pigments in the chloroplasts
- Chlorophyll a
- Chlorophyll b
- Carotene
How many TP molecules are made from every one CO2 molecule?
2 TP molecules are made from 1 CO2 molecule
How many turns of the Calvin cycle are needed to produce one hexose sugar, why?
6 turns:
- Because 3 turns produces 6 molecules of TP, but because 5/6 of these molecules are used to regenerate RuBP, 3 turns only produces 1 TP
- A hexose sugar has 6 Carbons, so 2 TP molecules are needed to form one
- So 6 turns of the cycle are needed to produce 2 TP’s which make 1 hexose sugar
What are the optimum conditions for photosynthesis, in most plants?
- High light intensity (of a certain wavelength)
- Temperature (around 25 oC)
- Carbon dioxide at 0.4%
Describe why high light intensity is an optimum condition for photosynthesis
- Light is needed for energy in the LDR, so the higher the light intensity the more energy it provides
- Only certain wavelengths of light are used in photosynthesis: chlorophyll a, b and carotene only absorb the red and blue light in sunlight (green is reflected so plants look green)
Describe why temperature at around 25 oC is an optimum condition for photosynthesis
- Photosynthesis involves enzymes (ATP synthase, rubisco): If the temp falls below 10 oC the enzymes become inactive. But, if the temp exceeds 45oC they may start to denature.
- At high temps stomata close to avoid too much water loss - causes photosynthesis to slow because less CO2 can enter the leaf (used in LIR)
Describe why carbon dioxide at 0.4% is an optimum condition for photosynthesis
- CO2 makes up 0.04% of the gases in the atmosphere
- Increasing this to 0.4% gives a higher rate of photosynthesis, but any higher the stomata start to close
When could CO2 levels be a limiting factor of photosynthesis?
On a warm, sunny, windless day
When could light intensity be a limiting factor of photosynthesis?
At night
What is the saturation point?
Where a factor is no longer limiting the reaction - something else has become the limiting factor
How do agricultural growers manage the limiting factors of photosynthesis?
They create optimum conditions, in glasshouses or polytunnels
How do agricultural growers manage carbon dioxide conc. as a limiting factor of photosynthesis?
In glasshouses, growers add CO2 to the air. E.g this can be done by burning a small amount of propane in a CO2 generator
How do agricultural growers manage light as a limiting factor of photosynthesis?
In glasshouses, light can get through the glass. At night, growers use lamps
How do agricultural growers manage temperature as a limiting factor of photosynthesis?
- Glasshouses trap heat from sunlight, which warms the air.
- Heaters and cooling systems can also be used to keep a constant optimum temp
- Air circulation systems make sure the temp is even throughout the glasshouse
Why do agricultural growers create optimum conditions for plant growth?
To increase plant growth, which increases yield (and therefore profit)
What are the two types of respiration?
Aerobic (requires O2) and anaerobic (doesn’t require O2)
Describe stage one of glycolysis (phosphorylation)
- Glucose is phosphorylated using a phosphate from ATP. This creates 1 molecule of glucose phosphate and 1 molecule of ADP
- ATP is then used to add another phosphate, forming hexose bisphosphate
- Hexose bisphosphate is then split into 2 molecules of triose phosphate (TP)
Is glycolysis anaerobic or aerobic?
Anaerobic
How many carbons does glucose have?
6