5 - Translation Flashcards
1
Q
what are the 3 segments of a mrna strand?
A
leader UTR
reading frame
Trailer UTR
2
Q
structure of a ribosome?
A
small [40] and large [60] sububit
large unit made up of T and A sites
3
Q
what are the t and a binding sites of a ribosome?
A
t - peptidyl trna binding site
where Meth binds w/ first trna
a - aminoacetyl trna bs
where 2nd aa with trna binds
4
Q
how does tran become charged?
A
enzyme: amino-acetyl trna synthase
theres a specific one [20] for each aa
5
Q
how do amino acids attach to a trna?
A
- an aminoacid bound to ATP binds to AATSynthase
- Atp hydrolyses to AMP
- AA bound to AMP
- trna attached to AATSynthase
- covalent bond form between trna [hydroxyl g] and aa [carboxyl g]
- AMP released
- trna and aa released
6
Q
initiation and termination of translation?
A
- mrna arrives at ribosome
- small subunit scans for methionine
- charged trna with meth attaches to p site on large subunit
termination
- release factor binds to A site when trailer stop codon
reached
7
Q
role of peptidyl transferase?
A
cause release of polypeptide from the trna in ribosome
