2 - Structure and Rep of DNA

Question 1

what is a nucelotide and side?

Answer 1

tide- pentose guar.], phosphate and base

side - everything but the phoshphate

Question 2

how to identify 5’ and 3’ end?

Answer 2

5’
has the phosphate attached to the 5’ sugar

3’
OH group attached to 3’ sugar

Question 3

what are the enzymes involved in dna repl?

Answer 3

helicase - breaks h bonds and unwinds strands

single strand binding protein - holds dna strands open to prevent h bonds reforming

primase - lays down rna primer and adds ribonucleoside triphosphates

DNA polyermerase - attaches to primer and adds bases
runs from 5’ to 3’

exonucleases
- cuts the rna primer out

ligase - joins together dna ends to form PD bonds