5- Photosythesis, Respiration And energy Transfer, Nutrient Cycles Flashcards
What does the lower epidermis do
Protects plants form water loss infection and injury
What is the photosynthesis equation (symbol)
6H2O + 6CO2 —> C6H12O6. + 6O2
In photosynthesis, light energy is first changed into ….
Energy and then into …… energy
Bond
Chemical
What are the 3 main stages of photosynthesis
Capturing light energy by photosynthetic pigments
Light dependent reaction
Light independent reaction
What are the products of the light dependent reaction
Reduced NAPD
ATP
o2
What are the 2 district regions in a chloroplast
Grana
Stroma
Describe the grana
Stacks up to 100 disc like structures called thylakoids
Some thylakoids have tubular extensions wich join up thylakoids to adjacent grana (called lamella)
Where is chlorophyll
In the thylakoids
Describe the stroma
Fluid filled matrix
Contains starch grains
What are the 2 raw materials of photosynthesis
H2O
Co2
Where does the light dependent reaction occur
Thylakoids membrane
Where does the light independent reaction occcur
Stroma
What are the products of the light independent reaction
Glucose
The light dependent reaction s involves the capture of Light, the energy is used for what 2 purposes
Add an inorganic phosphate to ADP making atp
Photolysis - H+ ions and OH - ions made when water is split by light
Oxidation results in energy …..
Given out
Reduction results in energy …
Being taken in
Describe the making of ATP in the light dependent rweaction
When a chlorophyll molecule absorbs light energy, it excites a pair of electrons within thus chlorophyll molecule.
They leave the chlorophyll molecule
The electrons that leave are taken up by an electron carrier, having lost a pair of e- they have been oxidised
The elctron carrier has been reduced
The electrons are now passed along a number of electron carriers in a series of oxidation and reduction reactions
These electron carriers form a transfer chain that is located in the thylakoids memebran e
Each new carrier is at a slightly lower energy level than the previous one so the electrons lose energy at each stage
Thus energy is used to combine and inorganic phosphate molecule with an ADP to make atp
What is photosytemt 2 called
Photosytem p680
Describe photolysis
The loss of electron that happen when light strikes the chlorophyll molecule leave it short of electrons
In order for the chlorophyll to continue to absorb light energy electrons must be replaced
They are replaced by water splitting a water molecule using light energy
This photolysis of water also yields protons
The protons are taken up by an electron carrier called NADP
When it takes up protons it becomes reduced
Reduced NADP is then passed onto the next stage with the electrons form they chlorophyl molecule
What is the bi products of photolysis
Oxygen
What is the photolysis equation
2H2O —>. O2 + 4H+. + 4e-
Give a summary of the chemiosmotic theory
Each thylakoids is in an enclosed chamber into which protons are pumped form stroma through proton carried
Energy to drive thsubcomes form e- released by photolysis’s
Photolysis also releases protons which inc in conc. in thylakoids space
Overall creates / maintain a conc gradient of portions across the thylakoids memebrane. High in thylakoids space low in stroma
The protons can only pass the thylakoid membrane through ATP synthase channels, the rest of the membrane is impermeable to protons, these channels form small granulesnon membrane surfaces AKA staked granules
As protons pass through these atp synthases channels cause changes to the structures of the enzymes which catalyses ADP + pi to atp
Chloroplasts adaptations
Thylakoids - large Sa - for attachment of chlorophyll electron carrier and enzymes that carry out light dependent reaction
Network of proteins in grana that hold chlorophyll molecules in a very precise manor that allows maximum absorbtionnofnlight
Granal mambranes have atpnsythase channel which catalysed atp production- their apps selectively permeable allowing a proton gradient
Chloroplasts have dna and ribosomes so they can quickly and easily manufacture proteins
Who discover the light independent stage of photosynthesis, how?
Melvin Calvin
Used c14 and traced
Describe the light independent stage of photosynthesis
CO2 diffuses through stomata and dissolves in water mesophyll, diffuses through cell surface membrane of plant cell, moves through cytoplasm and diffuses into chloroplast stroma
In stroma CO2 reacts with RUBP (5c ) which is catalysed by RuBiSCo
Reaction between CO2 and RuBP. Produces 2x GP (3c)
Reduced NADP and ATP are used to reduce GP into two times GAIP (3c)
NADP reform to go back to light dependent reaction to be reduced again and except more protons
Some TP molecules converted into organic substances such as starch cellulose lipids, nucleotides amino acids
Most TP molecules are used to make/regenerate RuBP using ATP
How many photosytstema are in a chloroplasts
2
What are the 2 photosytems
1 700 (absorbed light bast at 700nm)
2 680 (absorbed light best at 680nm)
Carbohydrates produced by photosynthesis and not used straight away, how are they stroedb
As starch grains in the stroma
What 3 things is the energy form photoionisation of cholorphyll used for
Making ATP from ADP and pi, this is called phosophorylation
Making reduced NADP from NADP
Splitting water into protonsm electrons and water ( pholysis)
What 2 types of phosphorylation does the light dependent stage inculde
Non cyclic
Cyclic
(Each of these process Hve differnt products )
What does non cyclic phosophorylation produce
ATP
Reduced NADP
O2
What does photosytem 1 do in the light dependent stage
Light is a doves by PS1 high excite electrons again to an even higher energy level
Final the electrons are transferred to NADP aloft with protons from the stroma to form reduced NADP
What does cyclic photolhosphorylation produce and why
ATP
Cyclic photophosphorylation only used PS1
It’s called cyclic becasue the electrons form the chlophyll molecule arnt passed onto NADP
But are passed back to PS1 via electron carriers
This means the electrons are recycles and can repeatedly flow through PS1
This process doesn’t produce any reduced NADP or O2 - it only proves a small amount of ATP
What is the Calvin cycle also known as and why
Carbon dioxide fixation
Because carbon from co2 is fixed into an organic molcule
How are carbohydrates ( hexose sugars ) made using TP. And GP
Joining 2 TP togetehr and larger carbohydrates are made by joining hexose sugars in differn ways
How are lipids made using TP. And GP
Made using glycerol which is synthesised from TP and fatty acids which are synthesised from GP
How are amino acids made using TP. And GP
Some amino acids are made form GP
How many molecules of TP are made earth cycle
2
For every 3 turns of the cycle …. Molecules of Tp are made
…/… of these TP molecules are used to regenerate…………
6
5/6
RuBP
For every 3 turns of the cycle only …. TP is procured that is used to make bexose sugar
1
6 turns of the Calvin’s cycle need …. ATP and …. Reduced NADP
18
12
Anaerobic respiration in plants and yeast produce what .
Ethanol and co2
And releases energy
Anaerobic respiration in humans procured what’s?
Lactate and reLEases energy
What makes ATP. A food energy source
ATP has Specific Properties that Make it a Good Energy Source
1) AT stores or releases only a small, manageable amount of energy at a time, so no energy is wasted as heat.
2) ‘s a small, soluble molecule so it can be easily transported around the cell.
3) Its easily broken down, so energy can be easily released instantaneously.
4) It can be quickly re-made.
3 It can make other molecules more reactive by transferring one of its phosphate groups to them (phosphorylation).
6 ATP can’t pass out of the cell, so the cell always has an immediate supply of energy.
What is a coenzyme
A molecule that aids the function of an enzyme
How do coenzymes work
Transferring a chemical group form one molecule to another
What is the coenzyme used in photosynthesis
NADP
NADP transfers hydrogen form one molecule to another
Examples of coenzymes used in respiration are
nad
Coenzyme A
FAD
………………,.,.,,.,.,.,.,.,,..,.,.,..,,..,.,,..,.,,..,,..,.,
NAD and FAD transfer hydrogen form one molecule to another -
A transfers acetate between molecules
Metabolic pathway definition
A series of small reaction controlled by enzymes
Phosphorylation Definition
Adding phosphate to a molcule
Photoionisation Definition
When light energy excited electrons in an atom or molecule, giving them more energy and causing them to be released, the release of electrons cause the atom or molecule to become a positively changed ion
Decarboxylation Definition
The removal of co2 form a molcule
Dehydrogenation Definition
The removal of hydrogen form a molcule
What do animals need energy for
Muscle contraction
Body temp regulation
Activate transport
DNA replication
Cell divison
Protein synthesis
Can a cell get energy directly form glucose , so
No
So energy released form glucose is used to make ATP
ATP diffused into part of cell where it is needed
When atp is hydrolysis
The chemical energy is released form where
The phosphate bond
What is the rate of photosynthesis effected by
Light intensity
Co2
Temperature
What is the law of limiting factors
At any given moment the rate of physiological process is limited by the factor that is at the least favourable value
Why is it hard to quantify photosynthesis
Level of co2 0.04%
Products of photosynthesis - hard to measure o2 in terrestrial plants
Temp changes - east to implement by rates of changes are difficult to quantity
When light is the limiting factor in photosynthesis the rate is a
Ways …….
Directly proportional to the light intensity
What is the light compensation point
It exists at the maximum amount of co2 absorbed responds to the o2 produced
Any further increase in rate would need inc co2
What are only certain wavelengths used in photosynthesis
Photosynthetic pig,ends chlorophyll a/b and carotene only absorbed red and blue light in the Sunlight
What is the optimum temp around for plants
25
At what temperatures are plants negatively effected and why
Photosynthesis involves enzymes (eg. ATP synthase and rubisco) if temp falls below 10°c the enzymes become inactive, move than 45° than may start to denature
Also at high temps stomata close to avoid too much water, this causes photosynthesis to slow down because less co2 enter leave when stomata are close
What is the optimum co2 % for photosynthesis
0.4% ( any high the stomata start to close)
What happens if a plant has too much water
The soil becomes water logged which reduced uptake of minerals which is needed to make chlorophyll
What is a saturation point
Where’s factor is no longer limiting the reaction
Something else has become the limiting factor
How do agriculture growers create optimum conditions for photosynthesis
Greenhouses ( glass houses)
How is co2 con managed in a glasshouse
Co2 is added or the air, by burning a small amount of propane in a co2 generator
How is light managed in greenhouses
Light can get thought glass and lamps are used at night
How is temperature managed in glass houses
Glasshouses trap heat energy from sunlight which warms the air, heaters and cooling systems can also be used to keep a constant optimum temperature and air air circulation system make sure the temperature is even throughout the glass house
Where can simsialr techniques that glasshouses used be used
Polytunnels (made of polythene, under which plants can be grown)
How did Calvin work out the light independent reaction
Lollipop
Algae are grown under light in thin transparent lollipop
Radioactive C14 in the form hydrogencarbonate is injected
At intervals samples on the photosynthesising algae are dropped into the hot methanol to stop chemical reactions instantly
The compound in the algae are separated by two water chromatography
The radioactive compounds are identified
What is an ecosystem
All the organisms living in a particular area and all the non-living (abiotic) conditions
What is a producer
Organisms that make their own food
Some of the sugars produced during photosynthesis are used in …… for what
Respiration to release energy for growth
Except respiration what is the rest of the glucose made from photosynthesis used for
Other biological molecules such as cellulose (in plant cells walls) these biological molecules make up the plants biomass
What is biomass
The chemical energy stored in a plant
How is energy transferred through living organisms of an ecosystem
When organisms eat other organisms ,
What can biomass be measures in terms of
Mass of carbon that an organism contains or the dry mass of its tissue per unit area per unit time
Why is dry mass uses as a measure of biomass rather than wet mass
Water content of living tissue varies
How is biomass measures (method)
1) dry mass is the mass of the organism with the water removed
2) to measure dry mass, a sample of the organism is dries, oftern in an oven at low temps, the sample is then weighed at regular intervals, once mass is constant you know all water has been removed
3) if needed the result can be sacked up to give the biomass of the total population or the area being investigated.
What is a typical unit for dry mass
Kg m-2
The mass of the carbon present generally is …..% of the dry mass
50%
Why is it usefull to give biomass over a particular time period
Biomass changes over time
What is atypical unit for biomass over a time period
Kg m-2 yr-1
How can you estimate the amount of chemical energy stored in biomass
By burning the biomass in a calorimeter
The amount of heat given off tells you how much energy is in it