5. Flow cytometry Flashcards
1
Q
what are the 3 samples needed for fluorescent imaging
A
cell only control (no formed elements, checks autoflourescence), isotype control (checks specific and non-specific binding) and the actual test sample
2
Q
forward scatter
A
size
3
Q
side scatter
A
granularity
4
Q
why do routine fluorescent imaging
A
HIV patients - helper t cells affected
5
Q
which dyes to use for Th fluorescence
A
FITC - green, CD3
PE - yellow/red, CD4
6
Q
how to remove erythrocytes
A
lyse using ammonium chloride in PBS
