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Immunosero Lab (Prelim) > 5. Anti-streptolysin O > Flashcards

5. Anti-streptolysin O Flashcards

1
Q

Characteristics of streptococcal infection?

A

Gram + cocci chains

Non motile

No spore forming

Facultative anaerobe

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2
Q

Streptococcal infection but gaseous requirements is obligate anerobe?

A

Peptostreptococci

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3
Q

Group A Streptococci?

A

S. pyogenes

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4
Q

Virulence factor that is associated with hyaluronic capsule; pharyngitis

A

M proteins

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5
Q

Virulence factor that induce TNF alpha and IL-1 beta?

A

Streptolysin S

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6
Q

MOT of S. pyogenes?

A

Direct contact to respiratory droplets

fomites

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7
Q

Clinical infection?

A

Strep throat
Scarlet fever
Pyodermal infection
Necrotizing fasciitis
Streptococcal Toxic shock syndrome
Acute rheumatic fever

Post streptococcal glomerulonephritis

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8
Q

detect antibodies to streptolysin O enzyme

A

Anti-streptolysin O

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9
Q

Recent streptococcal infections from patients suspected of having acute rheumatic fever and

post-streptococcal glomerulonephritis following a throat infection

Indication of?

A

streptolysin O

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10
Q

Hemolytic method for lab diag of streptolysin O?

A

ASO classic procedure

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11
Q

PRocedure for ASO classic procedure?

A

Dilute serum
Add SLO reagent
Incubate
Add RBC indicator

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12
Q

How do you report ASO classic procedure?

A

Titer with no hemolysis in Todd unit

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13
Q

What is considered moderately elevated in ASO classic procedure for young adults?

A

240 Todd Unit

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14
Q

What is considered moderately elevated in ASO classic procedure for Child?

A

320 Todd Unit

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15
Q

What is the limitations for ASO Classic procedure?

A

Streptolysin reagent and RBC reagent are not stable

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16
Q

What is the alternative or to make up the limitations for ASO classic procedure?

A

Nephelometry

17
Q

What is the unit for Nephelometry?

A

International Unit

18
Q

When will the Streptolysin O increase after infection?

A

1 to 2 weeks

19
Q

When will Streptolysin O peak following the symptoms?

A

3 to 6 weeks

20
Q

Percentage of antibody response to acute rheumatic fever

A

85%

21
Q

Is ASO titers helpful in individuals with skin infections?

A

False

22
Q

Why ASO titers is not use in skin infections?

A

Streptolysin O is not high in skin infection

23
Q

Procedure for ASO Latex test kit?

A

Latex particle w/SLO + serum

24
Q

What is the positive indicator for ASO latex test kit?

A

Agglutination

25
Q

Normal value for ASO latex test kit?

A

Less than or equal to 200 IU/mL

26
Q

Which of the following is not part of the preparation of Assay?

A.) Allow all reagents and samples to warm to room temp after use

B.) Gently mix the reagents before use; Avoid foaming

C.) Gently Mix the latex reagent before each use to ensure homogeneity

A

A. Should be “Before” use not after

27
Q

Which of the following is not part of the Qualitative procedure?

A.) Bring all reagents and specimens to cold temp

B.) Place one drop (50uL) of the positive control and Negative control and 50 uL of the Patient serum into separate circles on the glass slide

C.) Shake the ASO latex reagent gently and add 2 drop on each circle next to the sample to be tested and control

D.) Mix well using a disposable stirrer spreading the mixture over the whole test area and tilting the slide gently.

E.) Agitate for about 5 minutes with the rotator or by hand and observe

A

A.) should be room temp

C.) One drop (ALL One drop in this procedure)

E.) Agitate for about 2 minutes

28
Q

What is the dilution factor for Semi-quantitative procedure?

A

1:2 (2)

29
Q

What is the composition for Semi-Quantitative procedure?

A

0.25 mL serum/tube + 0.25 mL of saline

(ALL 0.25 mL)

30
Q

Which of the following is not part of the Semi Quantitative procedure?

A.) Serum to be titrated is serially diluted in 0.9g/L

B.) Place on drop of positive and negative on slide

C.) Dilute the control

D.) Place 1 drop (50uL) of each serum dilution individually in successive circles on the slide

A

C.) Never dilute control

31
Q

True or False

The serum ASO titer can be defined as the highest dilution showing a positive result

A

TRUE

32
Q

Formula for Approximate ASO level (IU/mL)

A

ASO titre =

Highest dilution with positive reaction X Reagent sensitivity (200 IU/mL)

Immunosero Lab (Prelim) (5 decks)

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