2. Serial and Compound Dilution

Question 1

Dilution uses different dilution factors?

Answer 1

Compound dilution

Question 2

Dilution uses same/Common dilution factors

Answer 2

Serial Dilution

Question 3

NOTE:

Answer 3

Sample
Dilution = ———
Sample + Diluent

Question 4

Material being diluted

Answer 4

Solute

Question 5

Medium making up the rest of the solution

Answer 5

Diluent

Question 6

The factor by which the stock solution is diluted

may be expressed as the ratio of the volume of the fial diluted solution to the initial volume removed from the stock solution

Answer 6

Dilution factor

Question 7

NOTE:

Answer 7

Final volume
Dilution Factor = ——————————-
Initial volume

Question 8

Where is serial dilution is usually used in IS?

Answer 8

Antibody titer

Question 9

SAMPLE PROBLEM: You need to do a 1:500 dilution for a certain assay (Compound dilution)

Answer 9

0.1 mL + 49.9mL NSS

0.1mL
———- x 10
50 mL

1mL serum
= ——————–
500 mL

Question 10

NOTE: Think a number divisible for 500?

= 5 x 10 x10 = 500

Question 11

NOTE: NO DECIMAL in dilution (Multiply it by 10 or depends)