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BACTE MIDTERMS > 4.3 > Flashcards

4.3 Flashcards

1
Q

BIOCHEMICAL TESTS used for Mycobacterium

A

a. Niacin Accumulation
b. Nitrate Reduction
c. Catalase Test
d. Tween 80 Hydrolysis
e. Iron Uptake
f. Arylsulfatase
g. Pyrazinamidase
h. Tellurite Reduction
i. Urease

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2
Q

What is the result of Niacin Accumulation for M. tuberculosis?

A

Positive result: Yellow color

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3
Q

Reagents of Niacin Accumulation for M. tuberculosis?

A

Cyanogen Bromide and Aniline

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4
Q

Niacin Accumulation Performed on

A

L-J medium, cultures at least 3 weeks old and with at least 50 colonies

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5
Q

Most commonly used for M. tuberculosis

A

Niacin Accumulation

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6
Q

Nitrate Reduction test principles

A

Nitroreductase catalyzes the reduction of nitrate to nitrite

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7
Q

Nitrate Reduction test Reagents

A

Hydrochloric acid,
Sulfanilamide,
N-(1-naphthyl)enediamine dihydrochloride

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8
Q

Nitrate Reduction test Indicator

A

Zinc

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9
Q

Nitrate Reduction test Positive result

A

Red color

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10
Q

Nitrate Reduction test Positive organisms

A

M. kansasii,
M. szulgai,
M. fortuitum, and
M. tuberculosis

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11
Q

Measurement of the height of the oxygen bubble column formed by untreated enzyme

A

Semiquantitative Catalase Test

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12
Q

What are the results for the Semiquantitative Catalase Test

A

<45 mm of bubbles = Negative
45 mm of bubbles = Positive

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13
Q

What are the conditions for the Semiquantitative Catalase Test?

A

Addition of Tween 80 and hydrogen peroxide

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14
Q

Tests the ability of catalase enzyme to remain active after heating at 68°C for 20 minutes

A

Heat-stable Catalase Test

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15
Q

Heat-stable Catalase Test Positive result spp present

A

M. tuberculosis,
M. bovis,
M. gastri,
M. haemophilum,
M. marinum remain active

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16
Q

Tween 80 Hydrolysis principle

A

Lipase hydrolyzes Tween 80 into oleic acid and polyoxyethylated sorbitol

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17
Q

pH indicator used in Tween 80 Hydrolysis

A

Neutral Red bound to Tween 80

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18
Q

Tween 80 Hydrolysis Positive result

A

Pink color

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19
Q

Tween 80 Hydrolysis Positive organisms

A

M. asiaticum,
M. gastri,
M. marinum,
M. malmoense,
M. scrofulaceum,
M. flavescens,
M. terrae;
M. kansasii

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20
Q

Iron Uptake test principle

A

Converts ferric ammonium citrate to an iron oxide

21
Q

Iron Uptake test Positive result

A

Rusty brown colonies

22
Q

What does the Iron Uptake test distinguish

A

M. chelonae (-) from other rapid growers (+)

23
Q

What is the biochemical reaction for Arylsulfatase?

A

Breaks down phenolphthalein disulfate into phenolphthalein

24
Q

Arylsulfatase Positive result

A

Pink color

25
Q

Arylsulfatase Reagents

A

Tripotassium phenolphthalein sulfate and sodium bicarbonate

26
Q

Arylsulfatase Positive organisms

A

M. fortuitum complex,
M. chelonae,
M. xenopi,
M. triviale (3 days);
M. marinum and
M. szulgai (14 days)

27
Q

Pyrazinamidase activity principle

A

Hydrolyzes pyrazinamide to pyrazinoic acid and ammonia

28
Q

Pyrazinamidase activity Positive result

A

Red pigment formed with ferrous ammonium sulfate

29
Q

Pyrazinamidase activity Distinguishes

A

M. marinum from M. kansasii and

M. bovis from M. tuberculosis

30
Q

What is the biochemical result in the Tellurite Reduction test?

A

> reduction of colorless POTASSIUM TELLURITE to BLACK METALLIC TELLURIUM in 3 to 4 days

31
Q

Tellurite Reduction test Positive result

A

Black color within 3-4 days

32
Q

Tellurite Reduction test Distinguishes

A

MAC from other nonchromogenic species

33
Q

What species are able to reduce tellurite within 3 days

A

All rapid growers

34
Q

What is the Urease test used to differentiate?

A

Distinguishes M. scrofulaceum (+) from M. gordonae (-)

35
Q

Urease test Positive result

A

Pink to red color

36
Q

->differentiates MTB from the scotochromogens and MAC

A

Nitrate Reduction

37
Q

Nitrate Reduction operating principle

A

> incubated in 2 ml of sodium nitrate at 37? C for 2 hours

38
Q

> differentiates species of photochromogens,
nonchromogens, and scotochromogens

A

Tween 80 Hydrolysis

39
Q

What are the inhibitory tests used for the identification of mycobacteria?

A

NAP (p-nitroacetylamino-Bhydroxypropiophenone)

Thiophene-2-Carboxylic Acid Hydrazide (TCH)

Sodium Chloride Tolerance

Growth on MacConkey Agar

40
Q

is a precursor in the synthesis of chloramphenicol

A

NAP (p-nitroacetylamino-Bhydroxypropiophenone)

41
Q

NAP (p-nitroacetylamino-Bhydroxypropiophenone) test Selectively inhibits

A

M. tuberculosis complex

42
Q

NAP reagent

A

5 mg of NAP in BACTEC-NAP vial

43
Q

NAP Specimen

A

Colonies from agar or broth in BACTEC medium

44
Q

NAP Positive result

A

20% increase in growth index (indicates M. tuberculosis complex)

45
Q

What does Thiophene-2-Carboxylic Acid Hydrazide (TCH) test differentiate?

A

Distinguishes M. bovis (Sensitive) from M. tuberculosis (Resistant)

46
Q

What does the Sodium Chloride Tolerance test inhibit

A

Inhibits M. flavescens, M. triviale, and most rapidly growing Mycobacterium species

47
Q

Sodium Chloride Tolerance test component

A

5% NaCl in egg-based media

48
Q

What is the result of Growth on MacConkey Agar for M. fortuitum-chelonae complex?

A

Positive result: Growth in 5 days

Negative result: Group IV rapid growers do not grow on MacConkey agar

BACTE MIDTERMS (17 decks)

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