4 - LIPID CHEMISTRY

Question 1

are generally insoluble in water but readily soluble in organic solvents, such as chloroform or acetone

Answer 1

Lipids

Question 2

solubility test samples

Answer 2

Olive oil, Coconut oil

Question 3

solubility test solvents

Answer 3

Dichloromethane (CHCl2), Benzene (C6H6), Ethanol (C2H5OH), Carbon tetrachloride (CCl4), Water (H2O), dilute Sodium hydroxide (NaOH) solution

Question 4

Make a drop of olive oil on a piece of filter paper.
On the same filter paper, add a drop of Ethanol (C2H5OH) and a drop of Ether.
After a few minutes, note the drop made on the filter paper by holding it up to the light

Answer 4

Spotting Effect

Question 5

positive spotting effect test

Answer 5

translucent spot of lipid

Question 6

Samples: Olive oil, Coconut oil
To each of the test tube, add 1 mL of chloroform and shake very well.
Add dropwise Iodine (I2) solution until a yellow color is obtain.
Shake, note the number of drops of Iodine solution added on each test tube.

Answer 6

Test for Unsaturation

Question 7

Place 1 mL of coconut oil in an evaporating dish and heat strongly.
Note the irritating disagreeable odor

Answer 7

Acrolein Test

Question 8

This test is used for the detection of Cholesterol

Answer 8

Leibermann-Burchard Test

Question 9

dehydrating agent in Leibermann-Burchard Test

Answer 9

Acetic anhydride

Question 10

oxidizing agent in Leibermann-Burchard Test

Answer 10

Sulfuric acid

Question 11

positive Leibermann-Burchard Test result

Answer 11

bluish-green solution

Question 12

this test is used for the detection of Cholesterol

Answer 12

Salkowski Test

Question 13

positive salkowski test result

Answer 13

when the Cholesterol layer becomes red or green

Question 14

Green layer contains

Answer 14

bicholestadiene monosulfonate

Question 15

Red layer contains

Answer 15

bicholestadiene disulfonate

Question 16

Prepare three test tubes as follows:
1 – 1 mL water + drop coconut oil
2 – 1 mL water + 1 drop coconut oil + 1 drop Na2CO3 solution
3 – 1 mL water + 1 drop coconut oil + a pinch of soap

Answer 16

Emulsification of Fats