1 - PRECIPITATION OF PROTEINS Flashcards
disrupts hydrogen bonds of secondary and tertiary protein structure
Heat
Albumin is coagulated when heated to form
white precipitate
high molecular weight cations
Heavy metals
Albumin reacts with Ferric chloride (FeCl3) to form
yellowish precipitate
Albumin reacts with Silver nitrate (AgNO3) to form
white precipitate
Egg white is used as an antidote for
Lead (Pb) and Mercurial (Hg) poisoning
used in the cauterization of wound
Silver nitrate (AgNO3)
Albumin reacts with concentrated Nitric acid (HNO3) to form
white ring at the junction of two layers
Albumin reacts with concentrated Nitric acid (HNO3) to form white ring at the junction of two layers
Heller’s ring test
Albumin reacts with concentrated Sulfuric acid (H2SO4) to form ? ring at the junction of two layers
white
Albumin solution is added with ? until saturation to precipitate the protein
solid Ammonium sulfate ((NH4)2SO4)
The solution is filtered and the filtrate is added with
Biuret reagent
Albumin solution is added with saturated Ammonium sulfate ((NH4)2SO4) solution to precipitate the protein
Half-Saturation Test
Albumin solution is added with solid Ammonium sulfate ((NH4)2SO4) until saturation to precipitate the protein.
Full Saturation Test
high molecular weight anions
Alkaloidal reagents
