3 - CHROMATOGRAPHIC SEPARATION

Question 1

very useful technique in the separation and identification of components of mixture based on the differences in affinities for the stationary phase and the mobile phase

Answer 1

Chromatography

Question 2

Stationary phase of PC

Answer 2

Filter paper

Question 3

Mobile phase of PC

Answer 3

2 mL 2% Ammonia – 4 mL Isopropyl alcohol solvent

Question 4

sample of PC

Answer 4

Amino acid solution

Question 5

Visualization agent of PC

Answer 5

Ninhydrin

Question 6

important technique in the identification and separation of mixtures of organic compounds

Answer 6

Thin-layer Chromatography

Question 7

Stationary phase of TLC

Answer 7

Silica gel or Aluminum oxide

Question 8

Mobile phase of TLC

Answer 8

Butanol – Acetic acid – Water (4:1:5) solvent

Question 9

Sample of TLC

Answer 9

Amino acid solution

Question 10

Visualization agent of TLC

Answer 10

Ninhydrin