4 genetic information, variation and relationships between organisms Flashcards
kms
what are the properties of dna in eukaryotes
. molecules are longer and linear and histone bound
. dna wound to fit into the nucleus, histones support dna
. dna and histones coil tigjtly to form chromosomes
what are the propertiesof dna in prokaryotes
. dna molecules are shorter, circular and not histone/protein bound
. (mitochondria and choroplasts in eukaryotes also contain this type of dna)
what is a gene
. dna contains genes
. gene is a sequence of bases on dna which determine aa sequence of a polypetide
. does this by coding for functional RNA
what is a locus
. fixed position occupied by a gene ona particular dna molecule
whta si an allele
. genes exist in different forms
. different versions called alleles
. order of bases in each allele varies so code for different versions of the same polypeptide
what is triplet code
. a sequence of three dna bases that code for a specific amino acid
. a triplet is known as a codon
what is one codon
. one amino acid
explain the features of teh genetic code
. code is universal for all organisms
. non overlapping so each triplet is read separately
. degenerate - some aa have more than one triplet code
.
what are genome and proteome
. genome - all genes ina cell
. proteome - all proteins that a cell ca produce
What are Introns and exons
. Exons - sequences within a gene code for amino acid sequences. 
. Intro - non-coding sequences.
What are the features of mRNA and T RNA? 
. MRNA-single-stranded, linear chain, long chain, corresponds to genetic sequence of a gene, unpaired bases.
. tRNA - single-stranded folded into clover shape, hydrogen bonds, anticodon, amino acid attachment, or similar lengths. 
Explain polypeptide synthesis
. One dna strand acts as a template
. Complementary copies transcribed on mRNA in nucleus
.mRNA binds to complimentary base pairs using base pairing
. Ribosomes, mRNA axis template that is translated into chain of AA using complementary tRNA which carries specific AA which are linked together.
What is pre-mRNA and splicing?
. Happens in eukaryotic cells only.
. mRNA produced during transcription contains introns and exons (pre-mRNA)
. introns need removing because they don’t contain genetic info that can be translated to aa sequence
. spliced out of mRNA sequence with enzyme
. produces strand of mRNA containing exons only, known as mature mRNA
Describe how mRNA is formed by transcription in eukaryotes
. Hydrogen bonds between bases broke.
. One DNA strand acts as template.
. RNA nucleotides aligned by complementary base pairing
. Uracil base pairs with adenine
. RNA polymerase joins nucleotides.
. Phosphide bond form between adjacent nucleotides.
. Pre mena Sliced and introns removed.
Describe how polypeptide is formed by translation of mRNA
. mRNA attaches to ribosome.
. tRNA Anticodons bind to complementary mRNA codons (2 bind at same time)
. TNA brings a specific amino acid.
. AA joints together with ATP
. tRNA released.
. Ribosome moves along mRNA to form the polypeptide 
What is a Homologous chromosome?
. Chromosome with the same genes equals homologous
What is meiosis?
. Produces gametes (haploid) using two divisions.
. Meiosis one separates homologous chromosomes reducing cell from diploid to haploid
. Miosis two separates sister chromatids producing four genetically varied haploid cells
How does meiosis lead to variation and genetic diversity?
. Produces unique gametes that are genetically different.
. Crossing over (prophase) with formation of chiasmata
. Homologous pies of chromosomes associates formed bivalent, chiasmata forms, equal length of non sister chromatids are exchanged, produces new combinations of alleles
. Independent assortment of homologous chromosomes.
. In metaphase one homologous pairs lineup at either side of the equator, completely random which side, once separated one of each pair ends up in Daughter celL, shuffling leads to different combinations of maternal and paternal chromosomes in daughter cells.
. Random meeting with Gammy results in variation.
. Mutations.
What is a mutation?
. Are changes in base sequence of chromosomes resulting in a new allele and can rise spontaneously during DNA replication.
. Substitution, deletion, insertion 
What are substitution deletion and insertion mutations?
. Substitution – one base minimum change for another during replication, only affects one cold on surrounding triplet stay sage.
. Deletion - one base Left out in DNA replication, results in frames shift all codons after mutation or affected.
. Insertion – an extra based added during DNA replication resulting in frames shift, all codons after mutation affected. 
What does it mean that genetic code is degenerate
. Not obey substitutions cause change in sequence of encoded AA
. More than one codon code is the same AA
. Move my result in codon coding for same amino acid and t struc does not change
How could the change in a codon affect the genetic code
. Amino acid sequence changes
. Hydrogen/ionic/disulphide bonds between R group changes
. Tertiary structure changes
. Could make a S not complimentary to substrate so cannot form complexes