3.3.16 Chromatography

Question 1

Thin Layer Chromatography (TLC)

Answer 1

A plate is coated with a solid and a solvent moves up the plate

Question 2

Gas Chromatography (GC)

Answer 2

A column is packed with a solid or with a solid coated by a liquid, and a gas is passed through the column under pressure at high temperature.

Question 3

Column Chromatography (CC)

Answer 3

A column is packed with a solid and a solvent moves down the column

Question 4

How does Column Chromatography work

Answer 4

Separation depends on the balance between solubility in the moving phase and retention by the stationary phase. Measure retention time.
Moving phase - Solvent
Stationary phase - Solid powdered substance

Question 5

How does Gas Chromatography work

Answer 5

Separation depends on the balance between solubility in the moving phase and retention by the stationary phase. Measure retention time.
Moving phase - Solvent
Stationary phase - Solid, powdered substance

Question 6

What is GC-MS

Answer 6

Gas Chromatography Mass Spectrometry. Molecules are passed through gas chromatography and then put into mass spectrometer for identification. Much faster, more accurate.