3.2.1 Clinical Use Of Monoclonal Antibodies Flashcards
1
Q
What is an antibody?
A
Immunoglobulin secreted by B cells
2
Q
What is an antigen?
A
Foreign substance that stimulates antibody production
3
Q
Label the antibody
A
4
Q
What is an epitope?
A
Antibody binding site
5
Q
What is a paratope?
A
Antigen-binding site
6
Q
How are monoclonal antibodies used in clinical practise?
A
Diagnostics
Therapeutics
7
Q
What are monoclonal antibodies?
A
Antibodies which bind to the same epitope and are produced from a single B-lymphocyte clone
8
Q
How are monoclonal antibodies produced? Put pic in
A
- Immunise against a specific epitope on an antigen and then harvest the B-lymphocytes from the spleen of the mouse
- B lymphocytes then fused with immortal myeloma cell line not containing any other immunoglobulin-producing cells
- Resulting hybridoma cells cultured in vitro so only hybridomas survive
- Selected hybridomas are found making a specific desired clonal antibody
9
Q
What are two important features of monoclonal antibodies?
A
Specific
Can be targeted against almost any cell-surface receptor
