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SB/PMLS-1 UNIT 1 > [3.2] Materials in Clinical Laboratory > Flashcards

[3.2] Materials in Clinical Laboratory Flashcards

1
Q

A substance which something is made or can be made

A

Material

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2
Q

It is used for the reliable collection, separation, storage, and transport of blood.

A

Blood Bag

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3
Q

They are used to collect and separate the three different blood components (plasma, red cells, buffy coat).

A

Triple top and bottom blood bags

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4
Q

Each blood bag is composed of?

A

Anti-coagulant additive solution, primary bag, platelets transfer bag and donor needle gauge

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5
Q

It is a single-use, sterile, winged blood collection needle bonded to a flexible tubing with a lure connector. Used in phlebotomy procedures.

A

Blood Infusion Set

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6
Q

Blood Collection is done by __________
Blood transfusion is done by ___________

A

Medical Technologists
Nurses

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7
Q

What actions are necessary to do before blood transfusion?

A

Blood typing and Cross matching

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8
Q

It is used in forward blood typing procedures and blood banking

A

Blood Typing Sera

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9
Q

Type of blood typing sera:
Anti A:

A

Blue

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10
Q

Type of blood typing sera:
Anti B:

A

Yellow

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11
Q

Type of blood typing sera:
Anti D:

A

White

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12
Q

What are the two Blood Group System?

A

ABO Blood Group System and RH Blood Group System

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13
Q

Who developed the ABO Blood Group System?

A

Karl Landsteiner

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14
Q

Landsteiner Rule

A
  • A person does not have an antibody to his own antigen
  • Each personhadtheantibodytotheantigenthat he/she lacks
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15
Q

Where are the ABO Blood Group antigens located?

A

On the surface of the red blood cell (RBC)

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16
Q

Where are the ABO Blood Group antibodies located?

A

located in the plasma

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17
Q

It is used to collect samples of liquids, which
will flow up into the tubes against the effects of gravity in a process called capillary action.

A

Capillary Tube

18
Q

It is used to puncture the skin, and blood will go through the capillary tube

A

Lancet Needle

19
Q

Best site for skin puncture:

A

lateral or medial plantar surface of the heel, or third or fourth finger of the non-dominant hand

20
Q

A counting-chamber device originally designed and
usually used for counting blood cells.

A

Hemocytometer

21
Q

The manual method of counting blood cells for confirmatory testing

A

Hemocytometer

22
Q

Specific mixtures of nutrients and other substances that support the growth of microorganisms such as bacteria and fungi (e.g. yeasts and molds).

A

Culture Media/Growth Media

23
Q

The standard equipment used for routine venipuncture. It consists of a needle syringe, a tube holder, and an air-evacuated tube

A

Evacuated Tube System

24
Q

Type of Evacuated Tube that stores plasma (“unclotted” liquid portion of the blood)

A

Evacuated Tubes with Anticoagulant

25
Q

Type of Evacuated Tube that stores clotted liquid portion of the blood

A

Evacuated Tubes with no Anticoagulant

26
Q

A hollow needle commonly used with a syringe to
inject substances into the body or extract fluids from it

A

Hypodermic Needle and Syringe

27
Q

The gauge number of the needle is __________ proportional with / or to the size of the bore (hole)

A

inversely

28
Q

Needles with gauge numbers ranging from ______ are used for venipuncture; while needle with a ____ gauge number is used for pediatric patients

A

19-23
while
23

29
Q

Used to enhance contrast in samples, generally at the microscopic level

A

Staining Dyes

30
Q

What are the three types of stains? Differentiate each.

A
  1. Simple Stain- Only make us of a single stain
  2. Differential Stain- Used to differentiate and contrast specimen
  3. Special Stain- Used to stain specific part of the bacteria (e.g. flagella, spores, capsules) or specific parts of the body (e.g. collagen in calcium in tissues)
31
Q

Used to differentiate gram-positive from gram
negative bacteria

A

Gram Stain

32
Q

What are the four components of gram stain?

A
  • Crystal Violet Stain
  • Grams Iodine
  • Alcohol
  • Safranin
33
Q

It has a thick layer of peptidoglycan/PTG

A

Gram Postive

34
Q

It has a thin layer of PTG

A

Gram Negative

35
Q

A device used to apply pressure to a limb or extremity
in order to limit—but not stop—the flow of blood

A

Tourniquet

36
Q

A tourniquet should be _____wide and ______ long

A

1 in. wide and 18-20 in. long

37
Q

A thin, plastic stick with strips of chemicals on it. It is placed in the urine to detect abnormalities and check its chemical components

A

Urine Dipstick

38
Q

11 Parameters

A
  1. pH level
  2. specific gravity
  3. protein
  4. glucose
  5. ketone
  6. blood
  7. bilirium
  8. Urobilinogen
  9. Nitrite
  10. Leukocyte/WBC
  11. Ascorbic Acid
39
Q

Gives the relative percentage of each type of white
blood cell (neutrophil, eosinophil, basophil,
lymphocytes, monocytes). Helps reveal abnormal white blood cell populations

A

Differential Blood Count / Coulter Counter

40
Q

These needles are sued for both intravenous delivery
of fluids or drugs or for blood collection. Used for glutathione treatment; gauge: 25

A

Winged Infusion Set

41
Q

List of Special Stains
Differentiate each.

A

▪ Van gieson: stains collagen
▪ Periodic acid schiff: stains glycogen
▪ Oil red o: stains fat lipids
▪ Lead method: stains 5 nucleotidase enzyme
▪ Felipe & Lake: stains acetylcholinesterase
▪ Methyl green pyronin: stains RNA and DNA
▪ Feulgen Stain: stains DNA
▪ Alkali Fast green: stains histones & protamine
▪ Perl’s Pression Blue: stains ferric iron
▪ Turnbull’s: stains ferrous iron
▪ Vonkossa: stains calcium
▪ Wadefite: stains legionella pneumophila
▪ Levaditi: stains Spirochetes

SB/PMLS-1 UNIT 1 (4 decks)

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