[3.1] Equipment in Clinical Laboratory Flashcards

1
Q

Set of articles or physical resources, serving to equip a person or thing

A

EQUIPMENT

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2
Q

A machine used to carry out industrial and scientific
processes requiring elevated temperature and
pressure in relation to ambient pressure/temperature

A

Autoclave

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3
Q

Work Principle of Autoclave

A

121 degrees Celsius at 15 lbs.
pressure for 15 to 30 mins

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4
Q

Types of Autoclaves

A

*Pressure Cooker Type
* Horizontal Autoclave
* Vertical Autoclave
* Common Laboratory Autoclave
* Large Automatic Hospital Autoclave

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5
Q

This type of Autoclave is used in surgical operations

A

Large Automatic Hospital Autoclave

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6
Q

A device that uses centrifugal force to separate
various components of a fluid

A

Clinical Centrifuge

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7
Q

Clinical Centrifuge is commonly used to separate ______ and _______ from
_____________, and in washing ____________.

A

Serum and Plasma
from Cellular components and in washing RBC

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8
Q

What are the different components of blood?

A

Plasma
Cellular Components

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9
Q

This is the liquid portion of blood and 55% of Total Blood Volume

A

Plasma

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10
Q

What are the Components of plasma?

A

▪ 91% water
▪ 7% blood proteins (fibrinogen, albumin,
globulin)
▪ 2% nutrients (amino acids, sugar, lipids),
hormones (erythropoietin, insulin, etc.),
electrolytes (sodium, potassium, calcium, etc.)

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11
Q

Which of the following contain antibodies?
A. Plasma
B. Serum
C. Both

A

C. BOTH, Plasma, and Serum contain antibodies.

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12
Q

It is 45% of Total Blood Volume
and composed of:
▪ Buffy Coat
▪ Red Blood Cells

A

Cellular Components

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13
Q

It is the white portion seen in between plasma and RBC
* Composed of the white blood cells (7000-
9000 per cubic mm of blood), and platelets
(250,000 per cubic mm of blood)

A

Buffy Coat

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14
Q

It is about 5,000,000 per cubic mm of blood

A

Red Blood Cells

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15
Q

Production of Blood Components

A

Erythropoiesis- RBC/ erythrocytes
Leukopoiesis- WBC/ leukocytes
Thrombopoiesis- platelets / thrombocytes

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16
Q

It uses multiple lenses to enlarge the image of a sample

A

Compound Microscope

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17
Q

What are the two separate lens systems of the compound microscope?

A

Objective and Eyepiece, the product of which produces the final magnification.

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18
Q

Components of objectives
* Scanner
magnification:
color:

A

magnification: 4x
color: red

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19
Q

Components of objectives
LPO – low power objective lens
magnification:
color:

A

magnification: 10x
color: yellow

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20
Q

Components of objectives
HPO – high power objective lens
magnification:
color:

A

magnification: 40x
color: blue

21
Q

OIO - oil immersion objective lens
magnification:
color:

A

magnification: 100x
color: white/black

22
Q

Eyepiece - magnification:

A

10x

23
Q

Total Magnification=

A

magnification of objectives (x10)

24
Q

It is used to adjust the lateral separation of the eyepieces for each individual.
When it is properly adjusted, the user should be able to focus both eyes comfortably on the specimen and visualize one clear image.

A

Interpupillary Control

25
Q

It connects the eyepieces with the objective lens. The standard length is 160 mm, which, functionally, is the distance from the real image plane (eyepieces) to the objective lenses.

A

Optical Tube

26
Q

This provides a structural site of attachment for the / holds the revolving nosepiece.

A

Neck / Arm

27
Q

It is the main vertical support of the microscope.
The stage assembly, together with the condenser and base, is supported by the stand.

A

Stand

28
Q

Holds the objectives and allows for easy rotation from one objective lens to another.

A

Revolving Nosepiece

29
Q

Supports the prepared microscope slide.

A

Stage

30
Q

Adjustments can be incorporated into one knob or can be two separate controls

A

Focus Controls / Adjustments

31
Q

May be permanently mounted or vertically adjustable with a rack-and-pinion mechanism.
It gathers, organizes, and directs the light through the specimen.

A

Condenser

32
Q

It is located under the stage, which moves it along an x- or a y-axis.

A

Stage Controls / Stage X-Y Adjustment Knobs

33
Q

It is located below the condenser within the base.
and should always be open; when it is open, it allows a maximally sized circle of light to illuminate the slide.

A

Field Diaphragm

34
Q

A laboratory equipment used to heat samples

A

Dry Bath

35
Q

Electrical devices which use dry heat to sterilize

A

Hot Air Oven

36
Q

Device used to grow and maintain microbiological
cultures or cell cultures

A

Incubator

37
Q

A cutting tool used to produce extremely thin slices of material known as sections

A

MIcrotome

38
Q

It is classified as a simple microtome

A

Rocking Microtome

39
Q

Inventor of Rocking Microtome

A

Paldwell Trefall

40
Q

It is the most common microtome

A

Rotary Microtome

41
Q

Inventor of Rotary Microtome

A

Charles Minot

42
Q

It is the most dangerous microtome

A

Sliding Microtome

43
Q

Who developed the Sliding Microtome

A

Adams

44
Q

It is used for undehydrated tissues in a frozen state

A

Freezing Microtome

45
Q

Who developed the Freezing Microtome?

A

Queckett

46
Q

An instrument that measure the amount oh photons
absorbed after it passes through sample solution

A

Spectrophotometer

47
Q

What is the intensity of light absorbed after it
passes through a solution?

A

Photons

48
Q

A laboratory equipment made from a container filled
with heated water. Used to incubate samples in water at a constant temperature over a long period of time

A

Water Bath