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Immunosero Lab (Finals) > 3.1 COVID-19 > Flashcards

3.1 COVID-19 Flashcards

1
Q

When is the first cases of COVID?

A

8 December 2019

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2
Q

First report of 27 cases of pneumonia with unknown cause in Wuhan China

A

31 December 2019

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3
Q

China announced the identification of a novel coronavirus as the causative agent of the pneumonia outbreak

A

9 January 2020

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4
Q

Case of a traveler from Wuhan was confirmed in Thailand

A

13 January 2020

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5
Q

Wuhan was locked down

A

21 January 2019

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6
Q

The coronavirus spead to all 34 provinces across China

A

29 January 2020

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7
Q

WHO declared a PHEIC alert

A

30 January

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8
Q

WHO risk assessmnet increased to very high on the global level

A

28 Feb 2020

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9
Q

ICTV named virus SARS-CoV-2 and WHO named disease COVID-19

A

11 Feb, 2020

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10
Q

WHO defined COVID-19 as a pandemic

A

11 March 2020

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11
Q

39 year old female had symptoms of cough and sore throat, and Chinese national

A

Jan 25 2020

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12
Q

44-year-old male, had sumptoms of fever, cough, and chills symptoms. Chinese national

A

Jan 31, 2020

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13
Q

What are the 2 large genes of COVID 19?

A

ORF1a

ORF1b

(Open reading frame)

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14
Q

ORF1a and b encodes the 16 structural proteins of the virus

T or F

A

F

Nonstructural

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15
Q

Nonstructural proteins works on the transcription and replication of the virus

T or F

A

T

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16
Q

NSP3 = 3CL-protease
NSP5 = papsin-like protease

T or F

A

F

Baligtad

17
Q

Structural genes are the ones that encodes the structural proteins of the virus

T or F

A

T

18
Q

What protein helps in distinguishing virus similar to SARS-CoV-2?

A

Accessory protein

19
Q

Is a critical component of the COVID-19 response

A

Diagnostic testing

20
Q

Diagnostic testing is a critical component of the COVID-19 response, as it can be used to:

  1. Confirm infection in patients who fulfill COVID-19 clinical criteria
  2. Rapidly screen suspected cases
  3. Screen for infection in asymptomatic contacts of confirmed COVID-19 cases
  4. Determine exposure to the virus to understand the true extent of the outbreak, map the pandemic across counties and monitor trends

Which of the following is wrong?

A

None

21
Q

Are foreign molecular structures that can trigger an immune response

A

Antigen

22
Q

Is a protein made by the body’s immune system that can recognize and target an antigen for destruction

A

Antibody

23
Q

Use: to detect active SARS-CoV-2 infection

Target: Viral genetic material

Method: Amplification of small amounts of the viral genome until reaching detectable levels

Location: Lab

A

Molecular test

24
Q

Use: to detect active SARS-CoV-2 infection

Target: Viral proteins

Method: Capture antigens on a test strip causing color change

Location: Pont of care

A

Antigen tests

25
Q

Use: Assess past infection

Target: Antibodies that patients develop in response to infection

Method: Capture of antibodies on a test strip or in a test well causing color change, read manually or using an instrument

Location: Lab or point of care

A

Antibody tests

26
Q

SARS-CoV-2 antibody test used to detect IgM and IgG antibodies in human serum, plasma or whole blood in vitro

T or F

A

T

27
Q

SARS-CoV-2 antibody test : These immunoglobulins indicates the person’s immune response against the COVID-19 infection

T or F

A

T

28
Q

What is the priniciple of SARS-CoV-2 antibody test?

A

Lateral flow immunochromatographic assay

29
Q

The test uses anti-human IgM antibody, anti-human IgG and goat anti-rabbit IgG immobilized on a nitrocellulose strip

T or F

A

T

30
Q

In SARS-CoV-2 antibody test : 10uL of whole blood, plasma, or serum is used

T or F

A

T

31
Q

How many drops of buffer is used in SARS-CoV-2 antibody test?

A

3 drops (90uL)

32
Q

What is the specimen used in SARS-CoV-2 antigen test?

A

Nasopharygeal or nasal swab

33
Q

Both SARS-CoV-2 antigen and antibody test uses the lateral flow assay principle

T or F

A

T

Immunosero Lab (Finals) (4 decks)

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