[3.1] Biological Molecules Flashcards
Monomers & Polymers, Carbohydrates, Lipids, Proteins & Enzymes, Nucleic Acids, ATP, Water & Inorganic Ions
What are monomers & polymers?
- Monomers are the smaller units from which larger molecules are made.
- Polymers are molecules made from a large number of monomers joined together.
What happens in condensation & hydrolysis reactions?
CONDENSATION REACTION
- 2 molecules join together
- Forming a chemical bond
- Releasing a water molecule
HYDROLYSIS REACTION
- 2 molecules separated
- Breaking a chemical bond
- Using a water molecule
Give examples of polymers and the monomers from which they’re made.
- Polynucleotide (DNA or RNA) - made up of nucleotides.
- Polysaccharide (starch, cellulose, glycogen) - made up of monosaccharides.
- Polypeptide - made up of amino acids.
Are lipids polymers?
- Lipids are not polymers as they are not made up of repeating monomers.
- Instead lipids are known as macromolecules.
What are monosaccharides? Give 3 common examples.
- Monosaccharides are the monomers from which larger carbohydrates are made.
- Glucose, galactose and fructose are common monosaccharides.
What are disaccharides and how are they formed?
- Two monosaccharides joined together with a glycosidic bond
- Formed by a condensation reaction, releasing a water molecule.
List 3 disaccharides and the monosaccharides from which they’re made.
- Maltose - glucose + glucose
- Sucrose - glucose + fructose
- Lactose - glucose + galactose
Draw the structure of an alpha-glucose molecule.
Draw the structure of a beta-glucose molecule.
Draw a diagram to show how two molecules of alpha-glucose join together.
What are polysaccharides and how are they formed?
- Many monosaccharides joined together with glycosidic bonds
- Formed by many condensation reactions, releasing water molecules.
Describe the basic function and structure of starch.
FUNCTION OF STARCH
- Energy store in plant cells.
STRUCTURE OF STARCH
- Polysaccharide formed by the condensation of alpha-glucose molecules.
- Amylose - 1,4-glycosidic bonds, unbranched.
- Amylopectin - 1,4- & 1,6-glycosidic bonds, branched.
Describe the basic function and structure of glycogen
FUNCTION OF GLYCOGEN
- Energy store in animal cells.
STRUCTURE OF GLYCOGEN
-
Polysaccharide formed by the condensation of alpha-glucose
molecules. - 1,4- & 1,6-glycosidic bonds, branched.
Explain how the structures of starch and glycogen relate to their functions.
STARCH (AMYLOSE)
- Helical - compact for storage in cell.
- Large, insoluble polysaccharide molecule - can’t leave cell/cross cell membrane.
- Insoluble in water - water potential of cell not affected.
STARCH (AMYLOPECTIN) & GLYCOGEN
- Branched - compact so fits more molecules in small area.
- Branched - more ends for faster hydrolysis to release glucose for respiration to make ATP for the release of energy.
- Large, insoluble polysaccharide molecule - can’t leave cell/cross cell membrane.
- Insoluble in water - water potential of cell not affected.
Describe the basic function and structure of cellulose.
FUNCTION OF CELLULOSE
- Provides strength and structural support to plant/agal cell walls.
STRUCTURE OF CELLULOSE
- Polysaccharide formed by the condensation of beta-glucose molecules.
- 1,4-glycosidic bond - straight, unbranched chains.
- Chains linked in parallel by hydrogen bonds forming microfibrils.
Explain how the structure of cellulose relates to its function.
-
Every other beta-glucose molecule is inverted in a long, straight,
unbranched chain. -
Many hydrogen bonds link parallel strands, known as crosslinks, to
form microfibrils. - Hydrogen bonds are strong in high numbers.
- So provides strength to plant cell walls.
Name some reducing sugars and describe how to test for them.
Reducing sugars = monosaccharides, maltose, lactose.
- Add Benedict’s solution to sample.
- Heat in a boiling water bath.
- Positive result = green/yellow/orange/red precipitate.
(Darker precipitate indicates a higher quantity of sugar)
Name a non-reducing sugar and describe how to test for it.
Non-reducing sugar = sucrose
- Do Benedict’s test and stays blue indicates a negative result.
- Heat in a boiling water bath with acid (to hydrolyse into reducing sugars).
- Neutralise with an alkali such as sodium hydrogencarbonate.
- Heat in a boiling water bath with Benedict’s solution.
- Positive result = green/yellow/orange/red precipitate.
Describe the biochemical test for starch.
- Add iodine dissolved in potassium iodide to sample and shake/stir.
- Brown/orange to blue/black indicates positive result.
Suggest two methods to measure the quantity of sugar in a solution.
METHOD 1
- Carry out a Benedict’s test.
- Filter and dry the precipitate.
- Find the mass/weight.
METHOD 2
- Make sugar solutions of known concentrations - dilution series.
- Heat a set volume of each sample with a set volume of Benedict’s solution for the same time.
- Use colourimeter to measure absorbance of each known concentration.
- Plot calibration curve - concentration x-axis and absorbance on y-axis and draw line of best fit.
- Repeat Benedict’s test with unknown sample and measure absorbance.
- Read off calibration curve to find concentration associated with unknown sample’s absorbance.
Name two groups of lipids.
Triglycerides and phospholipids.
Describe how triglycerides form.
- 1 glycerol molecule and 3 fatty acids
- Condensation reaction
- Removing 3 water molecules
- Forming 3 ester bonds
Describe the structure of a fatty acid.
- Variable R-group - hydrocarbon chain which can be saturated or unsaturated.
- -COOH - carboxyl group
Describe the difference between saturated and unsaturated fatty acids.
- Saturated: no C=C double bonds in hydrocarbon chain; all fully saturated with hydrogen.
- Unsaturated: one or more C=C double bond in hydrocarbon chain which creates a bend/kink.
Describe the difference between the structure of triglycerides & phospholipids.
One of the fatty acids of a triglyceride is substituted by a phosphate-containing group (PO₄³⁻).
What is the function of triglycerides?
Explain how the properties of triglycerides are related to their structure.
FUNCTION
- Energy storage
HOW THE PROPERTIES OF TRIGLYCERIDES RELATE TO THEIR STRUCTURE
- High ratio of C-H bonds to carbon atoms in hydrocarbon chain
- So used in respiration to release more energy than same mass of carbohydrates.
-
Hydrophobic/non-polar fatty acids so insoluble in water
- So no effect on water potential of cell.
What is the function of phospholipids?
Explain how the properties of phospholipids are related to their structure.
FUNCTION OF PHOSPHOLIPIDS
- Form a bilayer in cell membrane, allowing diffusion of lipid-soluble (non-polar) or very small substances and restricting movement of water-soluble (polar) or larger substances.
-
Phosphate heads are hydrophilic
- Attracted to water so point to water either side of membrane.
-
Fatty acid tails are hydrophobic
- Repelled by water so point away from water and point towards the interior of the membrane instead.
Describe the test for lipids.
- Add ethanol, shake and then add water.
- A milky white emulsion indicates a positive test.
Draw and describe the general structure of an amino acid.
- COOH - represents a carboxyl group.
- H₂N - represents an amine group
- R = variable side chain/group
How many amino acids are common in all organisms? How do they vary?
There are 20 amino acids that are common in all organisms and they only vary in their side group (R).
Describe how amino acids join together.
- Condensation reaction.
- Removing a water molecule.
- Between carboxyl/COOH group on one amino acid and amine/NH₂ group of another.
- Forming a peptide bond.