302 Q Flashcards
Mannitol salt agar (MSA) only allows the growth of halophiles (salt-loving microbes). Among the halophiles, mannitol fermenters release acid that turns the pH indicator yellow; mannitol nonfermenters leave the medium red. Onto MSA you inoculate a halophilic mannitol nonfermenter and a nonhalophilic mannitol nonfermenter. Here the medium acts as a \_\_\_\_\_\_\_\_\_\_ medium. Differential Selective Selective and differential Enrichment
Selective
Which of the following forms of bacterial respiration yields the least amount of ATP? Anaerobic respiration. Fat oxidation. Glucose fermentation. Aerobic respiration.
Glucose fermentation.
Which of the following characteristics is not true of alpha haemolysis?
Complete haemolysis of RBCs (Red Blood Cells).
Narrow zone of clearing.
Clearing displays indefinite margin.
Produces a greenish zone.
Complete haemolysis of RBCs (Red Blood Cells).
Which of the following characteristics is not true of beta haemolysis? Sharply defined zone of clearing Colourless zone of clearing Narrow zone of clearing (<2mm) Complete haemolysis
Narrow zone of clearing (<2mm)
The fruiting body of a mushroom is called Basidiocarps Plasmodiocarps Ascocarps Sorocarps
Basidiocarps
What is the purpose of a Oxidase test?
What is the purpose of a Oxidase test?
To test for the presence of the enzyme catalase.
To distinguish oxidative from fermentative metabolism.
To test if a bacteria is an obligate anaerobe
To test for the presence of cytochrome C.
To test for the presence of cytochrome C
Mannitol salt agar (MSA) only allows the growth of halophiles (salt-loving microbes); nonhalophiles will not grow. Among the halophiles, mannitol fermenters will produce acid that turns the pH indicator yellow; mannitol nonfermenters leave the medium red. Onto MSA you inoculate a halophilic mannitol fermenter and a halophilic mannitol nonfermenter. In this case, the medium is acting as (a) \_\_\_\_\_\_\_\_\_\_ medium(s). Differential Selective Selective and differential Enrichment
Differential
Bat sites usually harbor the following primary pathogenic fungus:
Bat sites usually harbor the following primary pathogenic fungus:
Phaeoannellomyces werneckii
Madurella mycetomatis
Paracoccidioides brasiliensis
Histoplasma capsulatum
Histoplasma capsulatum
Anaerobic respiration is distinguished from aerobic respiration by:
Anaerobic respiration is distinguished from aerobic respiration by:
Molecules other than O2 act as terminal electron acceptors.
Net oxidation of substrates.
ATP production by substrate level phosphorilation.
All of the above.
Molecules other than O2 act as terminal electron acceptors.
The cytoplasm is _______ .
The cytoplasm is _______ .
A selectively permeable barrier composed of hydrophillic and hydrophobic fatty acids.
The substance in which the nucleoid, ribosomes & inclusion bodies
are suspended.
The site of protein synthesis.
All of the above.
The substance in which the nucleoid, ribosomes & inclusion bodies
are suspended.
. You inoculated and then incubated an oxidation/fermentation (OF) test over the past 5 days. You return to find that the top third of the media has transformed from green to yellow. From this you derive that the inoculated bacteria \_\_\_\_\_\_\_\_\_ . Contains the enzyme Oxidase Is a mannitol fermenter Utilises an oxidative metabolism Utilises a fermentative metabolism
Utilises an oxidative metabolism
Which culture media is used to isolate staphylococci?
Which culture media is used to isolate staphylococci?
Blood agar
Mannitol salt agar
Chocolate agar
MacConkey agar
Mannitol salt agar
. Mannitol salt agar (MSA) only allows the growth of halophiles (salt-loving microbes). Among the halophiles, mannitol fermenters release acid that turns the pH indicator yellow; mannitol nonfermenters leave the medium red. Onto MSA you inoculate a halophilic mannitol fermenter, a nonhalophilic mannitol fermenter, and a halophilic mannitol nonfermenter. Thus, the medium is acting as a \_\_\_\_\_\_\_\_\_\_ medium. Differential Selective Enrichment Selective and differential
Selective and differential
The bread mold Rhizopus stolonifer belongs to which of the following fungal divisions? Ascomycota Chytridiomycota Microsporidia Zygomycota
Zygomycota
Select the corrent order in which biofilms form:
Active dispersal, Development, Colonisation, Reversable adhesion, Irreversible adhesion.
Reversible adhesion, Irreversible adhesion, Colonisation, Development, Active dispersal
Development, Active dispersal, Colonisation, Reversable adhesion, Irreversible adhesion.
Reversible adhesion, Irreversible adhesion, Development, Colonisation, Active dispersal.
Reversible adhesion, Irreversible adhesion, Colonisation, Development, Active dispersal
Fungi can be distinguished from algae due to
Fungi can be distinguished from algae due to
the presence of a chitinous cell wall
the presence of a visable nucleus
the presence of a cellulosic cell wall
The absence of mitochondria
the presence of a chitinous cell wall
Fungi usually store the reserve food material in the form of
Fungi usually store the reserve food material in the form of
Starch
Glycogen
Lipid
Protein
Glycogen
Which of the following is not true of haemolysis?
Which of the following is not true of haemolysis?
Beta haemolysis produces a clear zone around the bacterial colony.
Alpha haemolysis produces greenish discolouration of RBCs around the bacterial colony.
Gamma haemolysis produces no colour change around the bacterial colony.
Haemolytic function is associated with the enzyme streptokinase.
Haemolytic function is associated with the enzyme streptokinase.
What are the three basic steps of conventional PCR?
What are the three basic steps of conventional PCR?
Denature, anneal, & strand displacement
Strand displacement, synthesis & release
Reverse-transcription, anneal & extend
Denature, anneal & extension
Denature, anneal & extension
Fungi are major causes of plant diseases. are pest organisms that have no commercial value Only (i.) is true. Only (ii.) is true Neither (i.) or (ii.) are true Both (i.) and (ii.) are true
Only (i.) is true
Which ingredient makes MacConkey agar differential? Peptone Agar Lactose Bile salts
Lactose
If you are designing an experiment involving a chemolithotrophic autotroph; which of the following would be most essential to maintaining growth of the organism?
A continual supply of abundant oxygen
A nutrient medium containing glucose
Keeping the cultures at 37°C
A source of reduced inorganic compound such as NH4
A source of reduced inorganic compound such as NH4
Which by-product of bacterial fermentation is responsible for dental disease?
Which by-product of bacterial fermentation is responsible for dental disease?
Lactic acid.
NADH.
Ethanol.
Pyruvate
Lactic acid.
Blood agar is often used to differentiate between:
Bacteria that absorb oxygen and those that do not.
Salt sensitive and non-salt sensitive microbes.
Types of hemolysis the bacteria may produce.
All of the above.
Types of hemolysis the bacteria may produce.