3. Vibrio, Campylobacter, & Helicobacter Flashcards
Vibrio is gram ()?, oxidase ()?
Gram negative, oxidase positive
Are vibrio motile or nonmotile, where are they found?
Motile, in water
Vibrio bacteria is classified into 1. Non- halophilic / 2. Halophilic. What does each of them include?
- Non- halophilic ~ V. cholerae
* halophilic ~ V. parahemolyticus / V. vulnificus
Is V.cholarae transmitted via a vector or just by drinking contaminated water?
Just by drinking contaminated water!
V. Chlorae is a gram negative () shaped bacilli? Capsulated or not?
Comma shaped, NON- capsulated
Is V.cholerae aerobic or anaerobic?
Aerobic .
What is the enrichment media of v.cholerae?
Alkaline peptone water.
What’s the selective media of v. Cholerae?
TCBS media (thiosulphate citrate bile sucrose agar)
What color colonies does v. Cholerae have ?
Yellow colonies (sucrose fermenting colonies
What’re the biotypes of v.cholerae?
Classical and El Tor
What’re the somatic (0) antigen structure?
V.cholerae; O1 antigen structure
Non O1V.cholerae: no O1 antigen
What is v.cholerae pathogenesity?
The causative agent of choler a in humans.
Causes irreversible activation of cAMP
Produces enterotoxin
V. Choleral non - 01 (wl out somatic antigen)
Causes mild, bloody diarrhea
May produce toxins
Can cause epidemics
Is v. Parahemolyticus salt-tolerant? And what does it cause?
It is salt-tolerant\halophilic vibrio.
Causes food poisoning. (Acute gastroenteritis)
Is v. Parahemolyticus transmitted through a vector or just by contaminated water?
It is related to ingestion of sea good (fish and shellfish) (there is a vector)
Is V. vulnificus a halophilic or non-halophilic vibrio?
Halophilic.
Acute diarrhea caused by v.vulnificus following consumption of?
Shellfish
Does campylobacter bacteria multiply in food (salmonellosis)?
No
C. Jejuni and c. Coli pathogenesis ?
Commonest cause of infective diarrhea / invasive disease
Complications of c. Jejuni and c. Coli?
- G1 hemorrhage
- Toxic megacolon
- Hus ( hemolytic uritic syndrome)
- Reactive arthritis (having an infection away from the original place of infection)
- Pyogensitonsiitis
What’s the selective media of C.jejuni and c.coli?
Charcoal - based media containing antibiotics (to prevent the growth of unwanted bacteria)
C. Jejuni and c-coli require microaerophilic environment for growth (high conc. Of CO2)
C jejuni is the commenest hydrolyse hippurate in 90-95% of infections.
What’s helicobacter pylori?
Gram negative spirally-shaped
Microaerophilic
Man (only resevoir)
Oral-oral or fecal-oral rout for transmission.
Helibacter pylori liveonly in?
Gastric mucosa .
