3) Microscope review

Question 1

Name the parts of the microscope responsible for magnification, and their typical magnification levels.

Answer 1

Ocular lenses (10x) and objective lenses (4x, 10x, 40x, 100x).

Question 2

What part of the microscope controls the amount of light reaching the specimen?

Answer 2

The iris diaphragm.

Question 3

Which part of the microscope moves the stage up and down for focusing?

Answer 3

The coarse focus knob (for large adjustments) and fine focus knob (for small adjustments).

Question 4

What is the purpose of the condenser lens?

Answer 4

To focus light onto the specimen, improving clarity and resolution.

Question 5

What is the function of the stage?

Answer 5

To hold the slide in place for examination.

Question 6

What are the two key principles of magnification and resolution?

Answer 6

Magnification: Enlarging the image of the specimen.
Resolution: The ability to distinguish two closely spaced objects as separate entities.

Question 7

What is the role of immersion oil in light microscopy, and when is it used?

Answer 7

Once a specimen is in focus with one objective lens, it remains nearly in focus when switching to another objective lens.

Question 8

What is Koehler illumination, and why is it important?

Answer 8

A method of adjusting the light path to ensure even and optimal illumination across the field of view. It improves contrast and resolution.

Question 9

List the steps for setting up Koehler illumination:

Answer 9

Focus on the specimen using the 10x objective lens.

Close the field diaphragm.

Adjust the condenser height to sharpen the field diaphragm edges.

Center the field diaphragm using the condenser screws.

Open the field diaphragm until it fills the field of view.

Question 10

What are some key practices for proper microscope ergonomics?

Answer 10

Adjust the chair height to align your eyes with the eyepiece.

Keep your neck and back straight while sitting.

Take regular breaks to avoid eye strain.

Question 11

Why is proper posture important during extended microscope use?

Answer 11

To prevent musculoskeletal strain and reduce fatigue.

Question 12

What are the steps to properly clean microscope lenses?

Answer 12

Use lens paper and a small amount of lens cleaner to gently clean lenses without scratching them.

Question 13

How should you store a microscope when not in use?

Answer 13

Lower the stage.

Set the lowest objective lens in place.

Cover the microscope with a dust cover.

Question 14

What is the purpose of regularly calibrating the microscope?

Answer 14

To ensure accuracy and proper functioning of the microscope for consistent results.

Question 15

Explain the part of the microscope: Nosepiece

Answer 15

A rotating turret that houses the objective lenses. The viewer spins the nosepiece to select different objective lenses.

Question 16

Explain the part of the microscope: Aperture

Answer 16

The hole in the middle of the stage that allows light from the illuminator to reach the specimen.

Question 17

Explain the part of the microscope: Body tube (Head)

Answer 17

connects the eyepiece to the objective lenses.

Question 18

Explain the part of the microscope: Arm

Answer 18

connects the body tube to the base of the microscope

Question 19

Explain the part of the microscope: Stage

Answer 19

The flat platform where the slide is placed.

Question 20

Explain the part of the microscope: Coarse adjustment knob

Answer 20

Brings the specimen into general focus.
Use:

Used with low-power objectives (e.g., 4x or 10x).

Not recommended for high-power objectives, as large movements can cause damage to the slide or lens.

Question 21

Explain the part of the microscope: Fine adjustment knob

Answer 21

Provides precise focusing to make the image sharp.
Use:

Essential for high magnifications (e.g., 40x, 100x).

Helps fine-tune the focus without overshooting or damaging the sample.

Question 22

Explain the part of the microscope: Stage adjustment knob

Answer 22

Moves the stage up and down

Question 23

Explain the part of the microscope: Base

Answer 23

supports the microscope and it is where light source is located.

Question 24

Explain the part of the microscope: Condenser

Answer 24

Gathers and focuses light from the illuminator onto the specimen being viewed.

Question 25

Explain the part of the microscope: Iris diaphragm

Answer 25

Controls the amount of light hitting the condenser

Question 26

Explain the part of the microscope: Field diaphragm

Answer 26

Located in the base of the microscope. Used to control the amount of light coming from the light source

Question 27

How do you calculate the Total Magnification?

Answer 27

To calculate the magnification, multiply the power of the ocular lens (10x) by the power of the objective.

Question 28

Explain the part of the microscope:
Eyepiece (Ocular lense)

Answer 28

The part where you look into.. DUH