3-Lymphomas Flashcards
What is the order of chain rearrangements in a maturing lymphoid cell?
Heavy (V -> D ->J ) then light (V -> J)
What are the CD’s on a B precursor before heavy chain rearrangement?
CD19, 34. (19 stays always, even when mature)
What are the CD’s on a B precursor after heavy chain rearrangement?
CD10 (and still 19)
What are the CD’s on a pre-B cell? Which chain rearrangements have taken place at this stage?
- CD20 (and still 10 and 19)
- both heavy and light chain rearrangements
Mature T cells (2 types) display what CD’s?
- Cytotoxic: CD 8, 3 (3 is a marker for all T cells)
- Helper: CD 4, 3
So, what markers does a mature B cell have?
Same as “pre-B” cell!
CD 20, (from final stage)
10, (only if in an active germinal center)
19 (from early precursor stage)
Now let’s think of it the other direction….
:)
What cells display CD 19?
precursor B & mature B
So all in all, what CD’s are on B precursors?
19, 34, then 10.
What cells display CD 20?
mature B
What cells display CD 10?
precursor & germinal center cells
What cells display CD 3?
All T
What cells display CD 4?
cortical thymocytes & Helper T
What cells display CD 8?
cortical thymocytes & cytotoxic T
What cells display CD 34?
myeloid and lymphoid precursors
Germinal centers of lymph node contain?
follicular dendritic cell meshwork (stains w/ CD21) and other cells…
What causes a lymph cell to become larger, and gain prominent nucleoli and more cytoplasm?
antigen exposure
T/F lymphomas can originate from a tissue other than lymph nodes?
T
A lymphoma lymph node looks histologically different from a REACTIVE lymph node how?
lymphoma has effacement of architecture
Most important prognostic feature in a non-Hodgkin lymphoma is?
histologic subtype
[in Hodgkin it is stage]
Which tissues are PRIMARY lymphoid tissues?
ones T or B’s mature in (thymus, marrow)
[secondary would be anything else]
As T cells mature they move which direction in thymus?
toward medulla
[pos then neg selection]
T/F the T cell receptor is expressed during T cell maturation in thymus?
T
Where is MALT found?
Waldeyer’s ring, Peyer’s patch, and bronchus
What do M cells do?
uptake antigen
What’s in PALS? What about in white pulp?
- T cells
- B cells
What’s in cords of bilroth?
macrophages
If red pulp of spleen expands, it’s due to?
blood issue (congestion, leukemia etc) [where white expansion would be lymph reaction or cancer]
Nodular lesion in spleen =?
Hodkin or diffuse B lymphoma (no mets cuz no lymph afferents and all those immune cells kill blood-borne mets!)
What does a centroblast do?
Matures in a germinal center to a large cleaved, then small cleaved, then centrocyte (plasma or memory cell). It moves to lighter zone as it matures.
Follicular dendritic meshwork stains with what CD?
21
[same as what EBV uses to enter B cells!]
What does a B cell do when if finds antigen?
-Becomes proliferating immunoblast in interfollicular area
-migrates to primary follicle, becomes a centroblast that undergoes somatic hypermutation/prolif to become plasma or memory cell (centrocyte).
[Tingible body macrophages eat the dead B’s]
etiology of lymphadenOPATHY?
unkown (lymphadenitis is known pathogen)
Asteroid bodies mean what?
granulomatous lymph node
T/F reactive lymphoid hyperplasia regresses w/ antibiotics?
F
[and node is tender!]
BCL-2 is important why?
anti-apoptotic. Find in follicular lymphoma. (BCL-6 is opposite. apoptotic)
Many centroblasts and few centrocytes means?
cancer
EBER is a stain for what?
EBV RNA
Geographic necrosis of lymph node=?
bartonella henselae
How do you dx toxoplasma?
IgM test. note proliferation of monocytoid B cells.
How do you dx toxoplasma?
IgM test. note proliferation of monocytoid B cells.
