2.7 MICRO-ORGANISMS AND APPLICATIONS Flashcards
HOW CAN MICRO-ORGANISMS BE SEEN?
With light from a microscope
WHAT IS THE PURPOSE OF USING ASEPTIC TECHNIQUES?
Ensure the microorganisms being investigated do not escape or become contaminated with an unwanted microorganism
WHAT IS USED TO GROW MICROBES?
Nutrient agar gel
WHAT IS AN AGAR PLATE?
Agar gel within a petri dish
WHAT IS THE PURPOSE OF THE PETRI DISH LID?
Prevents microbes from the air contaminating the culture and stops the escape of any microbes on the plate
WHAT IS THE USE OF THE INOCULATING LOOP?
To transfer bacteria to a prepared nutrient agar plate
WHAT MUST HAPPEN TO ALL EQUIPMENT BEFORE IT IS USED IN ASEPTIC EXPERIMENTS?
They must be sterilised to kill unwanted microbes
WHAT TEMPERATURE ARE INOCULATED AGAR DISHES INCUBATED AT?
25 degrees (at school) to encourage growth without growing pathogens
HOW IS THE LID OF THE PETRI DISH SECURES FOLLOWING INOCULATION?
With strips of adhesive
WHAT CONDITIONS DOES BACTERIA NEED TO GROW?
optimum temperature and pH
WHAT HAPPENS IF TEMPERATURE OF BACTERIA IS TO HIGH?
Enzymes denature and growth rates fall to zero
WHAT ARE ANTIBIOTICS?
medicines that help to cure bacterial disease by killing or preventing the growth of the infective bacteria
WHERE IS PENICILLIN PRODUCED?
In a fermenter
WHAT CONDITIONS MUST THE FERMENTER HAVE TO PRODUCE PENICILLIN?
- Sterile air as penicillin is an aerobic organism
- Cooling water jacket surrounding due to heat released by the penicillin fungus
WHY IS THE CONTENT OF THE FERMENTER CONSTANTLY STIRRED FOR THE PRODUCTION OF PENICILLIN?
To ensure nutrients are evenly distributed and no waste material builds up
