2.3 More Microscopy Flashcards
Define ELECTRON MICROSCOPY
microscopy using a beam of electrons to illuminate a specimen, able to produce images with a higher resolution than light microscopes
Magnification of electron microscopes
up to 500 000x resolution
What is TRANSMISSION ELECTRON MICROSCOPY
TEM
when a beam of electrons is emitted through a specimen and focused to produce an image
has the smallest resolving power of 0.5nm
What is SCANNING ELECTRON MICROSCOPY
SEM
when a beam of electrons is sent across the surface of a specimen, electrons are reflected back, collected and used to produce a 3D image
has a resolving power of 3-10nm
What is LIGHT MICROSCOPY?
the use of light and glass lenses to enable the user to see an object that has been greatly magnified
resolving power of 200nm