2.2 Magnification and Calibration

Question 1

Calculation for Magnification

Answer 1

magnification = image size / object size

Question 2

How to calibrate a light microscope

Answer 2

• put the stage micrometer in place and the eyepiece graticule in the eyepiece of the microscope
• get the micrometer scale in focus
• align the micrometer scale and the eyepiece scale
• count how many divisions on the eyepiece are equal to the number of divisions on the micrometer
• ie 10 eyepiece divisoins equal 70 micrometre divisions so one eyepiece division equals 7 micrometres