23 - Molecular Diagnostics

Question 1

oncogene vs tumor suppressor

Answer 1

oncogene pos regulates cell division - activated in cancer

tumor suppressor neg regulates cell division - inactivated in cancer

Question 2

Philadelphia chromosome associated stuff

Answer 2

BCR ABL gene
t(9;22)
p190 (ALL), p210 (CML), p230

Question 3

imatinib (Gleevec)

Answer 3

blocks bcr-abl tyrosine kinase activity to stop growth and induce apoptosis

Question 4

best test to detect BCR ABL

Answer 4

rt-PCR

Question 5

molecular markers in neuroblastoma

Answer 5

n-myc: poor prognosis high risk group

ALK (anaplastic lymphoma kinase) (F1174, F1245, R1275) - poor prognosis but good response to ALK inhibitor

Question 6

aCGH

Answer 6

array comparative genomic hybridization
way to look for copy number problems in cytogenetics - hybridize fluorescent probes to target and look at how much there is

Question 7

Ewing sarcoma translocation

Answer 7

EWS-FLI1 t(11;22)