2.1.2 - Biological molecules Flashcards
Define the term ‘polymer’ ?
Polymer - A long chain molecule composed of a large number of individual units called monomers bonded together in a repeating pattern
Define the term ‘monomer’ ?
Monomer - Individual molecules that make up a polymer
Define the term ‘condensation reaction’ ?
Condensation reaction - A reaction between two molecules resulting in the formation of a larger molecule and the release of a water molecule
Define the term ‘hydrolysis reaction’ ?
Hydrolysis reaction - The breakdown of a larger molecule into two smaller molecules requiring the addition of a water molecule
What elements are carbohydrates made of ?
C, H and O
What elements are lipids made of ?
C, H and O
What elements are proteins made of ?
C, H, N, O and S
What elements are nucleic acids made of ?
C, H, N, O and P
What are the properties of water ?
- Water is a metabolite
- Water is an important solvent
- Water has a high boiling point/ specific heat capacity
- Water has a high latent heat of vaporisation
- Water has a strong cohesion/ surface tension
Explain how water is a metabolite ?
Water is a metabolite in many chemical reactions such as condensation and hydrolysis reactions
Explain how water is a solvent ?
- Water is a solvent in which metabolic reactions occur
- It readily dissolves numerous substances and allows for effective transport of substances
Explain why water has a high boiling point/ specific heat capacity ?
- Due to hydrogen binds, more energy is required to raise the temperature of water
- This allows water to act as a buffer against drastic temperature change
Name examples where water acts as a buffer to drastic temperature change ?
- Majority of organisms are mostly made up of water buffering them against drastic temperature change
- Aquatic environments are temperature-stable as water acts as a buffer against drastic temperature change
Explain how water has a high specific latent heat of vaporisation ?
- Due to hydrogen bonds, more energy is required to overcome bonds between water molecules and change the state of water
- This allows water to act as a coolant
Name an example where water acts a coolant ?
When we sweat, more heat is used from our body to evaporate the sweat off our skin providing a cooling effect
Explain how water has a strong cohesion/ surface tension ?
- This supports columns of water in xylem of plant setting up a transpiration stream
- This provides surface tension when water meets air ( ie. allows water to act as a habitant and support small organisms such as pond skaters )
Define the term ‘monosaccharide’ ?
Monosaccharide - A single sugar molecule
Name examples of monosaccharides ?
- Glucose ( alpha and beta )
- Fructose
- Galactose
Define the term ‘polysaccharide’ ?
Polysaccharide - A long-chain polymer made up of a large number of sugar monomers / monosaccharides chemically joined together
What is a hexose monosaccharide ?
A monosaccharide composed of six carbon toms
Name examples of hexose monosaccharides ?
- Alpha glucose
- Beta glucose
Draw the displayed formula of Alpha and Beta Glucose ?
What is a pentose monosaccharide ?
A monosaccharide composed of five carbon atoms
Name examples of pentose monosaccharides ?
- Ribose
Define the term ‘disaccharide’ ?
Disaccharide - A molecule composed of two monosaccharides joined together by a glycosidic bond
Name examples of disaccharides ?
- Sucrose
- Maltose
- Lactose
Name the monosaccharides that the disaccharides sucrose, lactose and maltose are composed of ?
- Sucrose = fructose + glucose
- Lactose = galactose + glucose
- Maltose = glucose + glucose
Explain what happens during a condensation reaction between two monosaccharides ?
- During a condensation reaction, a hydroxyl group from one monosaccharide and a hydrogen atom from another monosaccharide is removed
- This leads to the elimination of a water molecule and the formation of a glycosidic bond
What is the name of the bon that forms between monosaccharides ?
A glycosidic bond
Explain what happens during the hydrolysis of a disaccharides ?
- During a hydrolysis reaction, a single water molecule is added to a disaccharide to break the glycosidic bond between the monosaccharides
- This results in two individual monosaccharides
Draw a diagram to represent the formation of a disaccharide and glycosidic bond ?
What is starch ?
A polymer of alpha glucose which is an energy store in plants
What are the two types of starch ?
- Amylose
- Amylopectin
Explain the structure of amylose ?
- Amylose is formed by alpha glucose molecules joining together only by 1-4 glycosidic bonds
- This forms a helix shape ( making it compact and less soluble than glucose )
Explain the structure of amylopectin ?
- Same as amylose and additionally some glycosidic bonds form between carbon 1 and 6 on two glucose molecules
- This results in a more branched structure ( which can be hydrolysed to release more glucose molecules )
Explain the effect of the helix shape of starch/ glycogen ?
The helix-shaped structure causes it to be more compact so more glucose can be stored in a smaller volume
Explain the effect of starch/ glycogen being branched ?
Each end can be hydrolysed to release glucose for respiration/ transport
Explain the effect of starch/ glycogen being insoluble ?
- Too large to leave the cell
- Does not affect the water potential of the cell/ cause cell lysis
Explain the effect of glycogen being more highly branched than starch ?
- This means it has more ends which can be hydrolysed to release more glucose at a faster rate
- This allows glycogen to combat animals higher metabolic rate which requires more glucose due to being mobile unlike plants
What is glycogen ?
A polymer of alpha glucose which is an energy store in animals
Explain the structure of glycogen ?
- It is helix shaped
- It is insoluble
- It is more highly branched than starch ( both amylose/ amylopectin )
What is cellulose ?
A polymer of beta glucose resulting in straight, unbranched chains
Explain the structure of cellulose ?
- Cellulose is a polymer of beta glucose resulting in straight, unbranched chains
- Each cellulose polymer cab form hydrogen bonds with parallel molecules
- Cellulose molecules are bundled into microfibrils which are grouped into fibres
Explain how the structure of cellulose aids it function ?
This results in the rigidity of the cell wall, providing mechanical strength which prevents cell lysis
What are the two different types of sugars ?
- Reducing sugars
- Non-reducing sugars
What are reducing sugars ?
- Sugars that can reduce another molecule/ donate electrons
- All monosaccharides and some disaccharides are reducing sugars
What are non-reducing sugars ?
- Sugars that cannot reduce another molecule/ donate electrons
Name the test used to identify reducing sugars in a sample ?
Testing using Benedict’s Reagent
What is Benedict’s Reagent ?
Copper ( II ) Sulphate
Describe the test for reducing sugars ?
1 ) Place sample in a boiling tube
2 ) Add an equal volume of Benedict’s Regent into the boiling tube
3 ) Heat the mixture gently in a electric water bath for five minutes
Explain how Benedict’s Reagent helps identify reducing sugars in a sample ?
- Reducing sugars react with Benedict’s Reagent causing Cu2+ ions to be reduced to Cu+ ions
- This causes a brick-red precipitate to form/ CuO
Explain how testing for reducing sugars is a quantitive test ?
- The more reducing sugars present, the more precipitate forms as more Cu2+ ions are reduced to Cu+ ions
- Therefore, colour seen will depend on concentration of reducing sugars present
Explain why non-reducing sugars will no produce a positive result when tested with Benedict’s Reagent ?
Non-reducing sugars don’t react with Benedict’s Reagent and therefore will produce a negative result
Describe how to test for non-reducing sugars ?
- Boil non-reducing sugars/ sample with dilute hydrochloric acid ( this will cause non-reducing sugars to be hydrolysed and break down into its monosaccharides/ reducing sugars )
- Test for presence of reducing sugars using Benedict’s Reagent
Describe the test for the presence of starch ?
- Add a few drops of iodine solution ( iodine dissolve in potassium iodide ) to the sample
- Mix it with the sample
What is a positive and negative result when testing for the presence of starch ?
- If it changes form yellow/brown to blue/black it is positive
- If it does not change it is negative