2.1.1e/f Microscope Calibrations

Question 1

When do we use eyepiece graticule & stage micrometer

Answer 1

if you want to know the size of your specimen

Question 2

What is the eyepiece graticule

Answer 2

The eyepiece graticule is fitted onto the eyepiece. It has numbers but no units

Question 3

What is the stage micrometer

Answer 3

It is a microscopic ruler on a special slide w an accurate scale, placed on the stage. The ruler is 1mm long, & divided into 100 divisions. Each division is 0.01mm or 10μm. It is used to work out the value of each of the divisions on the eyepiece graticule at a particular magnification

Question 4

What does EPU stand for

Answer 4

Eye piece unit

Question 5

see showbie for eg

Question 6

Step by step method to calibrate microscope

Answer 6

1. Line up the stage micrometer and eyepiece graticule whilst looking through the eyepiece
2. Count how many divisions on the eyepiece graticule fit into one division on the micrometer scale
3. Each division on the micrometer is 10pm, so this can be used to calibrate what one division on the eyepiece graticule is at that current magnification