2.1.1.2 Cell structure - (Microscopes)

Question 1

What are the common stains used with samples in light?

Answer 1

Methylene blue and eosin

Question 2

What is differential staining?

Answer 2

Its when you stain each structure differently so the structure can be easily identified within a single preparation.

Question 3

Difference between Wet mount and Dry Mount

Answer 3

Wet mount involve the specimen been in a liquid

Question 4

Definition of an eyepiece graticule

Answer 4

A measuring device. It is placed in the eyepiece of a microscope and acts as a ruler when you view an object under the microscope.

Question 5

Definition of stage micrometer

Answer 5

A precise measuring device. It is a small scale that is placed on a microscope stage and its used to work out the value of division on the eyepiece graticule at a particular magnification.

Question 6

Explain why some samples need to be stained before they can be viewed under a light microscope.

Answer 6

To prevent the object being viewed from appearing completely white if its completely transparent.

Question 7

Explain why a variety of different stains are used for staining samples?

Answer 7

Particular stains can be used to make particular parts of cells show up.

Question 8

What is the function of an eyepiece lens?

Answer 8

magnifies and focuses the image from the objective into the eyes

Question 9

What is the function of an objective lens?

Answer 9

collects light passing through the specimen and produces a magnified image

Question 10

what is the function of a condenser lens?

Answer 10

focuses the light onto the specimen held between the cover slip and the slide.

Question 11

What is the function of the iris diaphragm?

Answer 11

is closed slightly to produce a narrow beam of light.