21.1: Producing DNA fragments Flashcards
1
Q
Recombinant DNA
A
The DNA of two different organisms combined by isolating genes, cloning and transferring them
2
Q
Genetically modified organism
A
GMO
Resulting organism from recombinant DNA techniques.
3
Q
GMO also known as
A
transgenic organism
4
Q
Stages in making a protein using DNA technology of gene cloning and transfer
5
A
isolation insertion transformation identification growth/cloning
5
Q
Methods of producing DNA fragments
3
A
- conversion of mRNA to cDNA using reverse transcriptase
- using restriction endonucleases
- the ‘gene machine’
6
Q
Retroviruses
A
Are able to synthesise DNA from their RNA in a host cell. Using the enzyme reverse transcriptase
7
Q
cDNA
A
complementary DNA.
Made up of nucleotides, complementary to the mRNA.
