2. Microbiology and Smoking Flashcards
What papillae are present in the tongue
- Filiform
- Fungiform
- Foliate
- Circumvellate
What antibodies in GCF
IgA
IgG
What evidence supports the bacterial aetilogy of periodontal disease
- Clinical Observation
- Perio Dx in germ free animals
3, Observation of monoinfected animals - Experimental disease in animals and humans
- Plaque control in humans
- Epidemiological Studies
- Pathogenic potential of bacterial plaque
Discuss Loe’s work in 1965
Plaque causes gingivitis
14 - 21 days to occur
Resolves with OH
Discuss the non specific plaque hypothesis
The bacterial community causes the disease
Discuss the specific plaque hypothesis
Certain bacteria cause the disease
What is the bacterial flora in health
Gram + non motile streptococci
What is the bacterial flora in gingivitis
Gram + rods eg A Viscous
What is the bacterial flora in perio dx
Gram Negative
Increasing motility
S Sanguis, F Nucleatum
Bacterial Flora in ANUG
Spirochete - T Pallidium
Bacterial Flora in ANUP
50% are uncultivable
What bacterial flora in abscess
Gram - rods
What are the red complex bacteria
- T Forsythia
- T Denticola
- P Gingivalis
What are the virulence factor of A Actino
- Polysaccharide / LDS
- Leukotoxin
- Extracellular proteins
- GroEl heat shock protein
- Fimbriae
- Other membrane proteins
What are the bacterial flora in T Denticola
- LPS
- Flagellum
- Extracellular Proteolytic Enzyme
What are the bacterial flora in T Forsythia
- LPS
2. Extracellular proteolytic enzymes
What are the bacterial flora in P Gingivalis
- Capsule
- LPS
- Fimbriae
- GroEl heat shock proteins
- Haemaglutin’s
Smoking is a systemic risk factor for what
- COPD
- MI
- Stroke
- CVD
- Cancer
What study identifies smoking as a risk factor
Grossi
How does smoking increase risk for perio dx
Toxins Immunosuppression Increased bacterial adhesion Reduced oxygen tension Vasoconstriction Increased cytokines Reduced fibroblast proliferation
CONFIT BV
What effect does smoking have on Non Surgical trt
Increased residual pocket
Less reduction in PD
Reduced wound healing
Less pathogen reduction
What effect does smoking have on surgical treatment
Reduces wound healing Reduces grafting success Reduced CAL Reduced PD reduction Reduced root coverage
What effect does smoking have on impl success
No difference
What effect does smoking have on maintenance and retreatment
Reduced success
Increased refractory
Graded response
What are the 5 A’s of smoking cessation
- Ask
- Advise
- Assist
- Arrange
- Assess
Why do Koch postulates not apply
Can’t grow some bacteria in media
What are Kochs Postulates
- Organism found in the lesion
- Must be isolated and grown in culture on artificial media
- Inoculation causes similar lesions
- Micro Organism isolated from the lesion
What are the alternatives to Kochs postulates
- Assoc with the disease
- Elimination of the organism
- Host Response
- Animal pathogenicity
- Mechanism of pathogenicity
How does P Ginigvalis cause increased clotting
Proteolytic enzymes produced by P Ging activate prothrombin
What does CRP do and why is it important
Binds to damaged cells, fixes compliment and activates neutorphil chemotaxis