2. DNA virus Flashcards
Largest, Most complex DNA virus
Poxviridae
Causative agent of small pox and the first virus that was eradicated in 1976
Variola virus
Vector for introducing active immunizing genes, maybe the product of genetic recombination
Vaccinia
Example of disease in Poxviridae?
Monkeypox
Mousepox
Cowpox
Camelpox
Orf
Bacteria like that contain vaccinia: 90% protein; 5% lipid, and DNA 3
Poxviruses
In poxvirus, what are the 2 viruses that causes vertebrate infection?
Orthopoxvirus
Parapoxvirus
Viral DNA and several viral proteins within the core
Nucleosome
In poxvirus, what part of it causes irregular surface tubules?
Outer membrane
Enclosed in a lipid bilayer envelope containing virus-specific substances
Virion
What part of the host cell does Poxvirus replicate?
Cytoplasm
Poxvirus Intermiediate transcription and late transcription takes place in the?
Cytoplasm
The replication of viral DNA and synthesis takes how many hours for post infection
2-6 hrs
The Manifestation of poxvirus is can be seen in what place of the Body?
skin
What do you call the eosinophilic inclusions?
Guarnieri bodies
Where is the primary multiplication for smallpox?
Lymphoid tissue
Where is the secondary multiplication for smallpox?
Other organs (Liver)
Which part of the skin is affected by cytoplasmic inclusion by smallpox?
Stratum spinosum
When is mouth lesion ulcerate happens for smallpox?
6th to 9th day
This mark as the 2ndary acantholysis
Ballooning degeneration
What is the incubation period of Poxvirus?
10 to 14 days
In poxvirus, how many days to cause fever and malaise?
1 to 5 days?
In poxvirus, when will the crusts fall off?
2 weeks
In poxvirus, the lesion can be seen more on the ?
Face than the trunk
What is the BSL for Poxvirus?
BSL 4
What is the serology for Poxvirus?
Ab assays
When should Ab assay be done for Poxvirus?
1 week after using HAI, neutralization, ELISA, RINA, and IF
Face, arms, back that has small, pink, wart like tumors
Umbilicated papule
Molluscum Contagiosum
Molluscum Contagiosum incubation period?
up to 6 months
Molluscum Contagiosum can commonly be seen in what age group?
children > adults
HHV1 targets?
Waist Up
HHV2 targets?
Waist down (Genital Herpes)
HHV3 is also known as?
Varicella zoster virus
HHV4 is also known as?
Cytomegalo virus
HHV5 is also known as?
Epstein bar virus (Kissing disease)
Virus that is caused by Roseola infantum, Exanthema subitum
HHV6
This encodes many enzymes and persists indefinitely in the infected host, spherical, icosahedral
Herpesvirus
Viruse hat cause Roseola?
HHV7
Virus that causes Kaposi sarcoma associated Herpes virus
HHV8
In Herpesvirus, A capsid is surrounded by amorphous proteins called
Tegument
tegument protein-complexes with several proteins and activates initial viral gene expressio
VP16
What are the subfamily of Herpesvirus
Alpha
Beta
Gamma
Subfamily of herpes, that has a short, cytolytic and the latent infections is the neurons
Alpha
What are the HHV of alpha?
1
2
3
Subfamily of herpes, that has a long, cytomegalic and long, lymphoproliferative with latent infections in glands, kidney, and lymphoid tissue
Beta
What are the HHV in Beta?
5
6
7
Subfamily of herpes, it has a Variable, lymphoproliferative with the latent infections in lymphoid tissue
Gamma
What is the HHV of Gamma?
4
8
Generalities of Herpesvirus, where does it reside before reactivation?
Sensory ganglia
How long does it take for the replication of HSV?
18 hrs
Viral DNA of herpesvirus is synthesized with what mechanism?
Rolling circle mechanism / replication`
What is the HSV vrial late gene products?
Glycoproteins
Proteins that is the product of immediate early genes wich stimulate transcription of early genes
Alpha proteins
Protein that is the product of early genes
Beta proteins
Proteins that is the product of late genes which is the viral structural proteins
Gamma proteins
Most potent inducer of neutralizing antibodies of HSV glycoproteins
gD
C3b-binding protein of HSV glycoproteins
gC
Fc receptorl binds with Fc of IgG of HSV glycoproteins
gE
type specific and allows for antigenic discrimination between HSV-1 and HSV-2 of HSV glycoproteins
gG
What is the clinical infections for HSV 1 and 2?
Trigeminal ganglia
Sacral ganglia
What is the latent infection forr HSV?
Lifetime
An intranuclear inclusion bodies, multinucleated giant cells
Cowdry Type A
Primary infection for herpes?
Break skin
Retrograde axonal flow to dorsal root ganglion
Incubation period for Varicella zoster virus?
10 to 21 days
Primary viremia fro Varicella zoster virus?
Bloodstream and replicates
Where is the secondary viremia?
Liver and spleen
Shingles commonly infect what part of the body?
Head, Trunk, Neck
Most common complication in the elderly related to Shingles?
Post herpetic neuroglia
An asymptomatic congenital disease is the most common cause of congenital infection with the largest genetic content of human herpesvirus?
Keratogenic
Cytomegalovirus
An oncogenic lymphoproliferative virus that B cell is the main target?
Epstein bar virus
Atypical lymphocytes seen in patients with mononucleosis
Downey cells
Seldom causes disease in rhesus monkeys
Neurologic disease is the striking feature of this virus
Herpes B virus
In the outer surface, what containcs in the inner nucleoplasmid and in viral genome?
HBsAG
In the outer surface, what containcs L strand?
Circular DNA
In the outer surface, what containcs S strand?
Minus strand
Lipoprotein envelope, surrounds an inner nucleocapsid core particle HBcAG
HBsAG
Not detectable in the serum unless the virion is treated with detergent
HBcAg
Smallest of known human pathogens?
Requires HBsAg coat for transmission
Hepatitis D virus
Use as a viral vector for astrazeneca vaccine?
Adenovirus
What adenovirus cause Respiratory diseases?
Adenovirus 3, 7, 21
(3 x 7 = 21)
What adenovirus cause Respiratory diseases?
Adenovirus 3, 7, 21
(3 x 7 = 21)
What adenovirus cause GI disease?
Adenovirus 40 and 41
Used as viral vector for COVID vaccines?
Adenovirus
Smalles DNA animal virus that causes erythema infectiosum, Transient aplastic crisis, Pure red cell aplasia, and hydrops fetalis?
Parvovirus B19
What are the 3 virus in Papovavirus
Papillomavirus
Polyomavirus
Vacuolating virus
Oncogenic virus that consist warts and cervical cancer?
Papillomavirus
Oncogenic virus the causes hemorrhagic cystitis and progressive mulifocal leukoenceohalopathy?
Polyomavirus
A DNA virus that has the potential to cause tumors in animals but most often persist as a latent infection?
Simian Vacuolating virus