(2) Flashcards
1
Q
a growth medium in which the amount and/or identity of at least one ingredient is unknown.
A
undefined media
2
Q
What is the common media used to maintain bacterial cultures?
A
Nutrient broth & nutrient agar
3
Q
What sources are necessary for growth media?
A
-they are formulated from sources that supply carbon & nitrogen in a variety of forms - amino acids, purines, pyrimidines, monosaccharides to polysaccharides, & various lipids
4
Q
How long and at what temperature must media be autoclaved before it is considered sterile?
A
15 mins at 121 degrees Celsius
5
Q
How are inoculating instruments sterilized?
A
By incinerating them in the Bunsen burner flame
