1.8 factors affecting enzyme action

Question 1

what is the effect of temperature on enzyme activity? describe the shape of the graph.

Answer 1

as temperature increases, rate of enzyme activity increases as particles
- fastest rate at optimum temperature
- rate decreases when over optimum temperature as enzymes are denatured

Question 2

what is the effect of pH on enzyme activity? describe the shape of the graph.

Answer 2

as pH increases, rate of enzyme activity increases
- fastest rate at optimum pH
- rate decreases when over optimum pH as it alter charges of R-group & disrupt tertiary structure bonds, so it doesn’t bind to substrates

Question 3

what is the effect of substrate concentration on enzyme activity? descirbe the shape of the graph.

Answer 3

as substrate concentration increases, rate of enzyme activity increases
- more collisions with enzymes
- reaction rate remains constant when substrate concentration increases further, as all enzyme active sites were occupied