1.7 - Growing and investigating bacteria Flashcards

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1
Q

What can be used for laboratory investigations of bacteria?

A

Pure cultures on non-pathogenic bacteria

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2
Q

Why might bacteria be used in a laboratory investigation?

A

To find the effects on antibiotics on bacteria

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3
Q

Why do investigations into bacteria require uncontaminated cultures?

A

Other bacteria could grow, like pathogens

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4
Q

Where could contamination come from in a laboratory investigation of bacteria?

A

Skin, the air, soil or the water in the air

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5
Q

What is the liquid or gel you give to a bacteria to culture them?

A

A culture medium, e.g agar jelly

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6
Q

What is contained in a culture medium? (4)

A

Nutrients
Carbohydrate as an energy source
Other minerals
Sometimes other chemicals

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7
Q

Once in a culture medium, what do bacteria require to culture?

A

Warmth and oxygen

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8
Q

What temperature should bacteria be incubated at in school laboratories?

A

25 degrees celsius

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9
Q

What temperature should bacteria be incubated at in the industry?

A

35 degrees celsius

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10
Q

What two things should you do to keep a culture of bacteria pure?

A
  • kill all bacteria on the equipment (pass metal loops through flame, boil solutions and agar, etc…)
  • prevent microorganisms from getting into equipment (don’t blow on inoculating loop to cool it, etc…)
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