1.7 Basic Separation Techniques

Question 1

A process whereby centrifugal force is used to separate solid matter from a liquid suspension

Answer 1

Centrifugation

Question 2

Supernatant - _______
Sediment- ________

Answer 2

Liquid

solid

Question 3

Parts of centrifugation includes:
- Head rotor
- Carriers
- Shields
- Motor
- control panel
- Breaking device
- Cover

Which of the following does not belong?

Answer 3

None

Question 4

Rule of thumb in Centrifuge: Placing the tube by exact positioning or by the exact opposite

t or F

Answer 4

T

Question 5

Purpose of the in Centrifue machine is for ________ Verification

a. Rotation
b. Speed
c. Specimen

Answer 5

b

Question 6

How to verify Speed in the centrifuge?

a. tachometer
b. Strobe light
c. All of the above
d. None of the above

Answer 6

c

Question 7

Force acting in the sample

a. Relative Centrifugal force
b. Revolution per minute

Answer 7

a

Question 8

Speed of the centrifuge

a. Relative Centrifugal force
b. Revolution per minute

Answer 8

b

Question 9

RCF Constant value?

Answer 9

)1.12 x 10 ^-5) r x rpm^2

Question 10

Centrifugal force increases the with increasing:

a. Speed
b. length of the radial arm

Which of this does not belong?

Answer 10

None

Question 11

Rotor and motor in Centrifuge is influence with RCF with Fictional force and Buoyant force

T or F

Answer 11

T

Question 12

How to obtain better separation?

a. Increase the sepeed
b Increae the time
c None

Answer 12

c

Question 13

w.o gel separator: how many mins for 1000g when it comes to centrifuge?

Answer 13

10-15 mins

Question 14

w/ gel separator: how many mins for 1000g - 1300g when it comes to centrifuge?

Answer 14

10-15 mins

Question 15

To protect biological specimens from famage caused by heating when centrigufed

a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge

Answer 15

a

Question 16

Use for terminally labile analytes

a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge

Answer 16

a

Question 17

Fixed at a angle

a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge

Answer 17

b

Question 18

About to 7000rpm

a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge

Answer 18

b

Question 19

Also known as Swining-bucket rotos have holders that swing outward in response to centrifugal force

a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge

Answer 19

c`

Question 20

Not capable of higher speed (3000rpm, 1700g)

a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge

Answer 20

c

Question 21

Which of the following is not a characteristic of horizontal Centrifuge

a. Specimen cups are in vertical position when at rest
b. The cups move to a horizontal position during centrifugation
c. Capable of higher speed
d. As the sample is centrifuged, the particles being sedimented travel down though the liquid to the bottom of the tube

Answer 21

c

Question 22

It uses a very high torque and low inertia motor to spread monolayers of cells rapidly across a special slide for critical morphologic studies

Answer 22

Cytocentrifuge

Question 23

Cytocentrifuge Advantage:

Small amount of sample is used producing e____ distributed cells

Answer 23

Evenly

Question 24

Which centrifuge is used when rapid centrifugation of solution is needed?

a. Horizontal-head
b. Fixed angle
c. Cytocentrifuge
d. None of the above

Answer 24

b

Question 25

Used to separate serum from a blood clot-used in place of centrifugation

a. Filtration
b. Dialysis

Answer 25

a

Question 26

Method of separating macromolecules from a solvent or smaller substances

a. Filtration
b. Dialysis

Answer 26

b