1.6 Types of Automatic pipet Flashcards
Uses a suction aspirate and dispense sample through a polyropylene tip
a. Air displacement
b. Positive displacement
c. Dispenser pipets
a
Piston mechanism: Air movement; relies on a piston for suction creation to draw a sample into a tip
a. Air displacement
b. Positive displacement
c. Dispenser pipets
a
Uses a glass capillary tip fitted with teflon tipped plunger
a. Air displacement
b. Positive displacement
c. Dispenser pipets
b
Tips are reusable
a. Air displacement
b. Positive displacement
c. Dispenser pipets
b
Tips are disposable
a. Air displacement
b. Positive displacement
c. Dispenser pipets
a
Piston mechanism: syringe like; operates by moving the piston in the pipet tip or barrel
a. Air displacement
b. Positive displacement
c. Dispenser pipets
b
Piston comes in contact with the liquid; no-air interface
a. Air displacement
b. Positive displacement
c. Dispenser pipets
b
For viscous and volatile liquids
a. Air displacement
b. Positive displacement
c. Dispenser pipets
b
Positive displacement pipet:
a. with no air cushion
b. Can pipety high density samples
c. Aspiration force remains constant
d. A, B, and C
d
Obtain from a common reservoir and dispense it repeatedly
a. Air displacement
b. Positive displacement
c. Dispenser pipets
c
Which of the following does not belong in dispenser pipet:
a. Bottle top
b. Motorized
c. Band-held
d. Attached to dilutor
e. None
e
Pipet Calibration:
Done by delivering and weighing a solution of known specific gravity
a. Gravimetric method
b. Photometric method
a
Pipets should be stored at what position?
a. Horizontal position
b. Vertical position
b
Pipets should be calibrated ________ a year
a. 1-2
b. 2-3
c. 3-4
d. 4-5
c
Pipet Calibration:
Uses spectophotometer
a. Gravimetric method
b. Photometric method
b