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ACTS (CC) > 1.1 Reagents and Reference Materials > Flashcards

1.1 Reagents and Reference Materials Flashcards

1
Q

Reagents are Pre-package which requires the addition of ________ or Buffer

a. Water
b. Acid
c. Alkaline
d. Control

A

a

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2
Q

The process of adding water or buffer to reagent

a. Reconstruction
b. Reconstitution
c. Reassemble
d. Relyophilization

A

b (Reconstituted Reagent)

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3
Q

Requires Mixing

a. Wet Chemistry
b. Dry Chemistry

A

a

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4
Q

In Wet Chemistry:

Ready to used reagent

a. Liquid Reagent
b. Lyophilized reagent

A

a

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5
Q

In Wet Chemistry:

Long shelf life

a. Liquid Reagent
b. Lyophilized reagent

A

b

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6
Q

In Wet Chemistry:

Short Shelf life

a. Liquid Reagent
b. Lyophilized reagent

A

a

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7
Q

In Wet Chemistry:

Requires the addition of Distilled water

a. Liquid Reagent
b. Lyophilized reagent

A

b (Made of powder)

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8
Q

In Wet Chemistry:

More stable

a. Liquid Reagent
b. Lyophilized reagent

A

b

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9
Q

In Wet Chemistry:

Creates Reconsituted Reagent

a. Liquid Reagent
b. Lyophilized reagent

A

b

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10
Q

What error commited if there is wrong process in making a reconstituted reagent?

a. Systematic
b. Random

A

Systematic error

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11
Q

Process of Freezing a material at -40 C or less and then subjecting it to a high vacuum. Very low temperatures cause the ice to sublimate to a vapor state. The solid non-sublimate material remains behind in a dried state

A

Lyophilization

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12
Q

Uses of Lyophilization:

  • Calibrations
  • Control materials
  • Reagents
  • Blank
  • Individual Specimens

Which of the following does not belong?

A

Blank

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13
Q

Reference laboratory for clinical chemistry?

a. EAC
b. Lung center of the Philippines
c. San Lazaro Hospital
d. National Kidney and Transplant Institute

A

b

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14
Q

Popular Analyzer that uses Dry chemistry during 1078 (Ortho products)

A

Vitros Analyzer

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15
Q

Vitros Analyzer introdurced _________ or Dry slide Technology

a. Thick film
b. Thin film

A

b

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16
Q

Layers of the thin film in Dry chemistry:

S_______
S ________
R ______
I __________
S ________

A

Spreader
Scavenger
Reagent
Indicator
Support

It first Spread then Looks for Something (Savenge) then it add something (Reagent) then once it shines (Indicator) we need to support it

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17
Q

Light source for dry chemistry?

A

Spectrophotometer

18
Q

Dry chemistry follows the Beers law when using spectrophotometry

T or F

A

F

19
Q

When getting a reagent what are the things that you should check?

  • Check the label
  • Check the lot #
  • Check for E________
A

Expiration date

20
Q

Expired Reagent can lead to?

a. False Positive
b. False negative
c. Shift
d. Trend

A

d

21
Q

What will you do when change in lot number?

a. Blank
b. Validation study
c. Control
d. Recalibration

A

b and d

22
Q

Reagent are stored in what temp?

a. 2-6 C
b. 2-8 C
c. 4-6 C
d. 4-8 C

A

b

23
Q

Reasons for using a calibrator/standard?

  • Change in the reagent ____ number
  • Reagent c______ is out of range
  • when performing method E_______and technical v________
  • ## When establishing a r_________ range
A

Lot

control

Evaluation and Validation

Reference

24
Q

How many LT sample are required for you to establish reference range?

a. 20
b. 40
c. 120
d. 25

A

c

20 - Verify
40 - Estimate

25
Q

Medtech must know:

  • Chemicals
    -Standards
  • Solutions
  • Buffer
  • Water requirements

Which of the following does not belong?

A

None

26
Q

Reagent Deterioration:

  • T_______ and Precipitation
  • Reagent fails to meet L__________ claims
  • Fails to recovcer c______ values in the stated range
  • M_________ has penetrated the vial and caking has occured
A

Turbidity

Linearity

control

moisture - Indication of expired

27
Q

If a medtech commits a pipetting error, what error is that?

a. Random
b. Systematic

A

a

28
Q

What is wrong when reagent is new but linearity has an error?

a. Machine error
b. Expired Reagent
c. Pipetting skills
d. Temp

A

c

29
Q

Interfering Substances for reagents:

  • Anticoagulants
  • Drugs and other substances
  • ____________________

a. Pyridoxal potassium
b. Pyridoxal phosphate
c. Pyridoxal phosphatase

A

b

From diluent water w/ Microbial growth

30
Q

Sample morphology

Bilirubin = ________
HgB = _____
Lipids = ______

A

Icteric

Hemolyzed

Lipemic

31
Q

Reportable range: 3 - 500 uL
Reference Range: 10 -20 uL
Patient results 400 uL

Release or not?

A

Release

since it is within the reportable range

32
Q

Performance characteristics of Reagents:

Also known as reportable range (AMR - Analytical measurement range)

a. Linarity
b. Sensitivity
c. Specificity
d. Precision
e. Comparison of new mtd

A

a

33
Q

Performance characteristics of Reagents:

Ability to detect positive indiv

a. Linarity
b. Sensitivity
c. Specificity
d. Precision
e. Comparison of new mtd

A

b

34
Q

Performance characteristics of Reagents:

Ability to detect true neg

a. Linarity
b. Sensitivity
c. Specificity
d. Precision
e. Comparison of new mtd

A

c

35
Q

What is the statistical test if there is significant difference? (Comparing more than two means)

a. T-test
b. ANOVA
c. Z-test
d. F test

A

b and d

If comparing only two means = T test

36
Q

Two commercial serum controls were assayed 20 times

a. Within run precision
b. Run to tun precision

A

a

37
Q

The lower SD and CV, ________ Precision and _________ Random error

a. Higher, lower
b. Lower higher
c. Higher, higher
d. Lower, lower

A

a

38
Q

2 Commercial serum controls were assayed for 5 consecutive days

a. Within run precision
b. Run to tun precision

A

b

39
Q

Control:

Values are determined by each individual laboratory for methods or instruments

a. Assayed control
b. Unassayed control

A

b

40
Q

Control:

Values are assigned by manufacturer

a. Assayed control
b. Unassayed control

A

a

ACTS (CC) (10 decks)

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