1.1 Reagents and Reference Materials Flashcards
Reagents are Pre-package which requires the addition of ________ or Buffer
a. Water
b. Acid
c. Alkaline
d. Control
a
The process of adding water or buffer to reagent
a. Reconstruction
b. Reconstitution
c. Reassemble
d. Relyophilization
b (Reconstituted Reagent)
Requires Mixing
a. Wet Chemistry
b. Dry Chemistry
a
In Wet Chemistry:
Ready to used reagent
a. Liquid Reagent
b. Lyophilized reagent
a
In Wet Chemistry:
Long shelf life
a. Liquid Reagent
b. Lyophilized reagent
b
In Wet Chemistry:
Short Shelf life
a. Liquid Reagent
b. Lyophilized reagent
a
In Wet Chemistry:
Requires the addition of Distilled water
a. Liquid Reagent
b. Lyophilized reagent
b (Made of powder)
In Wet Chemistry:
More stable
a. Liquid Reagent
b. Lyophilized reagent
b
In Wet Chemistry:
Creates Reconsituted Reagent
a. Liquid Reagent
b. Lyophilized reagent
b
What error commited if there is wrong process in making a reconstituted reagent?
a. Systematic
b. Random
Systematic error
Process of Freezing a material at -40 C or less and then subjecting it to a high vacuum. Very low temperatures cause the ice to sublimate to a vapor state. The solid non-sublimate material remains behind in a dried state
Lyophilization
Uses of Lyophilization:
- Calibrations
- Control materials
- Reagents
- Blank
- Individual Specimens
Which of the following does not belong?
Blank
Reference laboratory for clinical chemistry?
a. EAC
b. Lung center of the Philippines
c. San Lazaro Hospital
d. National Kidney and Transplant Institute
b
Popular Analyzer that uses Dry chemistry during 1078 (Ortho products)
Vitros Analyzer
Vitros Analyzer introdurced _________ or Dry slide Technology
a. Thick film
b. Thin film
b
Layers of the thin film in Dry chemistry:
S_______
S ________
R ______
I __________
S ________
Spreader
Scavenger
Reagent
Indicator
Support
It first Spread then Looks for Something (Savenge) then it add something (Reagent) then once it shines (Indicator) we need to support it
Light source for dry chemistry?
Spectrophotometer
Dry chemistry follows the Beers law when using spectrophotometry
T or F
F
When getting a reagent what are the things that you should check?
- Check the label
- Check the lot #
- Check for E________
Expiration date
Expired Reagent can lead to?
a. False Positive
b. False negative
c. Shift
d. Trend
d
What will you do when change in lot number?
a. Blank
b. Validation study
c. Control
d. Recalibration
b and d
Reagent are stored in what temp?
a. 2-6 C
b. 2-8 C
c. 4-6 C
d. 4-8 C
b
Reasons for using a calibrator/standard?
- Change in the reagent ____ number
- Reagent c______ is out of range
- when performing method E_______and technical v________
- ## When establishing a r_________ range
Lot
control
Evaluation and Validation
Reference
How many LT sample are required for you to establish reference range?
a. 20
b. 40
c. 120
d. 25
c
20 - Verify
40 - Estimate