16 – ATP synthesis & transport

Question 1

ATP synthase Harness … to…

Answer 1

Harness proton-motive force to make ATP

Question 2

how does complex V work?

Answer 2

Orange part – rigid/doesn’t turn/can be deformed

Green part – turns

Question 3

hwo does green part turn?

Answer 3

Turns by Brownian motion

Question 4

explain the orange part

Answer 4

it has 2 channels-protons can only go half way & displace side chain from arginine 210 to the start of the second channel&then the proton can leave

Question 5

how does the turning generates ATP?

Answer 5

Rotation of 𝛾 subunit – pushes β subunit through 3 state cycle

-𝛾 subunit = asymmetrical & when it turns - it deforms the β subunit

-ADP can enters
-ATP formed
-ADP held -not yet forced to form ATP

turns again:
-ADP held
-ATP leave
-ATP formed

Question 6

ATP escape mitochondrial matrix via

Answer 6

ATP/ADP antiporter

Question 7

how does ATP/ADP antiporter work?

Answer 7

exchange transporter powered by difference of concentration of 2 things on each side - when making ATP, ADP decreases inside