1.4 Enzymes Flashcards

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1
Q

What is an enzyme

A

Biological catalyst
Protein
Specific to a substrate
Can be used over and over again as not used up in the reaction

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2
Q

What is a substrate

A

The material that an enzyme will bind to - will be broken down by enzyme or built up by enzyme
E.g starch

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3
Q

What factors can affect enzyme activity

A

PH
Temperature
Enzyme concentration

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4
Q

What is the active site

A

The part of the enzyme that the substrate will bind to and form an enzyme-substrate complex

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5
Q

What happens to enzyme activity at low temperature

A

The enzyme activity is low
Due to enzymes and substrate having low kinetic energy so less Enzyme substrate complexes can be formed and less product is made

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6
Q

What is meant by optimum temperature or pH

A

This is the temperature or pH that the enzyme has the highest rate of production

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7
Q

What happens to enzyme activity if the temperature goes above optimum temperature

A

The enzyme activity starts to rapidly decrease
This is due to enzyme becoming denatured

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8
Q

What is meant by denatured

A

When the active site of the enzyme becomes changed or damaged and can no longer form an enzyme substrate complex
Desaturation happens due to high temperature or changes in pH above or below optimum pH

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9
Q

What happens to the activity of Enzymes when the pH changes from optimum pH

A

The enzyme becomes denatured
This can happen if the pH goes below or above optimum pH

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10
Q

In the digestive system where is the enzyme amylase produced

A

Salivary gland
Pancreas
Small intestine

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11
Q

In the digestive system where is protease produced

A

Stomach
Pancreas
Small intestine

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12
Q

In the digestive system where is lipase produced

A

Pancreas
Small intestine

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13
Q

What does amylase break down in digestive system

A

Amylase breaks down starch to sugars

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14
Q

What does protease break down in digestive system

A

Protease breaks down proteins to amino acids

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15
Q

What does lipase break down in digestive system

A

Lipase breaks down lipids or fats to fatty acid and glycerol

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16
Q

What is meant by digestion

A

The breaking down of large insoluble molecules to small soluble molecules
eg fats to fatty acid and glycerol
proteins to amino acids
starch to sugars

17
Q

What is assimilation

A

When the digested food eg glucose enters the blood and travels to the LIVER via the HEPATIC PORTAL VEIN
at the liver it is either stored or distributed round the rest of the body

18
Q

Where does food get absorbed in the digestive system

A

In the small intestine (ileum)

19
Q

Give an example of a commercial use of enzymes

A

Biological detergent

20
Q

What is the advantage of using biological detergent

A

Clean clothes at lower temperature - ECONOMIC - less money heating water (electricity needed)
- ENVIROMENTAL - less fossil fuels burnt to produce the energy to make electricity

21
Q

What is an inhibitor

A

A molecule that will fit the activie site - it will inhibit or prevent the proper substrate entering and combining with the active site so the rate of reaction will decrease

22
Q

What is the name of the model used to describe the joining of the enzyme and substrate

A

Lock and Key model