1.2 Carbohydrates and monosaccharides Flashcards
List the 2 most common features of monosaccharides.
sweet tasting
soluble
No.of carbon atoms - name - molecular formula
3 - Triose - C₃H₆O₃
4 - Tetrose - C₄H₈O₄
5 - Pentose - C₅H₁₀O₅
6 - Hexose - C₆H₁₂O₆
7 - Heptose - C₇H₁₄O₇
What is the above table an example of?
Monosaccharides.
What are carbohydrates as a monomer unit?
Monosaccharides
What do pairs of monosaccharides combine to form?
disaccharides
What do monosaccharides form when combined in much larger numbers?
polysaccharides
Glucose + Glucose combine to form?
maltose
glucose + fructose combine to form?
sucrose
glucose + galactose combine to form?
lactose
how are monosaccharides joined to create a polysaccharide?
by glycosidic bonds that were formed by condensation reactions
When monosaccharides join what molecule is removed?
What is the reaction therefore called?
Water.
condensation reaction.
How do we test if a disaccharide is a reducing sugar?
Benedicts solution
How do we know if a disaccharide is non-reducing when using Benedicts solution?
the colour of the solution does not change when the benedicts reagent is used
What must be done to a disaccharide in order for it to be detectable as a non-reducing sugar?
must be hydrolysed into its monosaccharide components through hydrolysis.
describe how to perform a benedicts test for non-reducing sugars, and what results show a positive outcome?
proceed with this test only after a negative benedicts test for reducing sugars
Add dilute hydrochloric acid to sample to be tested.
heat solution to boiling point using a water bath, this is the stage at which acid hydrolysis may occur.
cool the solution under cold water and add sodium hydrocarbonate to make the solution alkaline, as much powder as needed until fizzing stops.
Use universal indicator paper to ensure that the solution is alkaline, else the benedicts reagent will not work
Add benedicts reagent to solution and heat to boiling point.
positive result indicated by a brick red solution
How do you test for reducing sugars?
benedicts test